Versatile Bioconjugation Strategies of PEG-Modified Upconversion Nanoparticles for Bioanalytical Applications
| Autor: | Petr Skládal, Nadiia Velychkivska, Eliška Odstrčilíková, Uliana Kostiv, Daniel Horák, Matěj Pastucha, Hans H. Gorris, Zdeněk Farka, Ognen Pop-Georgievski, Matthias Jürgen Mickert |
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| Rok vydání: | 2020 |
| Předmět: |
Streptavidin
Bioconjugation Polymers and Plastics technology industry and agriculture Bioengineering 02 engineering and technology 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Combinatorial chemistry Polyethylene Glycols 0104 chemical sciences Biomaterials chemistry.chemical_compound chemistry PEG ratio Materials Chemistry Click chemistry Nanoparticles Azide 0210 nano-technology Ethylene glycol Maleimide Nanoconjugates |
| Zdroj: | Biomacromolecules. 21:4502-4513 |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.0c00459 |
| Popis: | Lanthanide-doped upconversion nanoparticles (UCNPs) display highly beneficial photophysical features for background-free bioimaging and bioanalysis; however, they are instable in high ionic strength buffers, have no functional groups, and are nonspecifically interacting. Here, we have prepared NIR-excitable UCNPs that are long-term colloidally stable in buffered media and possess functional groups. Heterobifunctional poly(ethylene glycol) (PEG) linkers bearing neridronate and alkyne or maleimide were attached to UCNPs via a ligand exchange. Streptavidin (SA)-conjugates were prepared by click reaction of UCNP@PEG-alkyne and SA-azide. Antihuman serum albumin pAbF antibody was modified with azide groups and conjugated to UCNP@PEG-alkyne via click reaction; alternatively, the antibody, after mild reduction of its disulfide bonds, was conjugated to UCNP@PEG-maleimide. We employed these nanoconjugates as labels for an upconversion-linked immunosorbent assay. SA-based labels achieved the lowest LOD of 0.17 ng/mL for the target albumin, which was superior compared to a fluorescence immunoassay (LOD 0.59 ng/mL) or an enzyme-linked immunoassay (LOD 0.56 ng/mL). |
| Databáze: | OpenAIRE |
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