RNA Interference Characterization of Proteins Discovered by Proteomic Analysis of Pancreatic Cancer Reveals Function in Cell Growth and Survival
| Autor: | Henrik S. Olsen, Steven M. Ruben, Candy Lee, Paul A. Moore, Katherine McKinnon, Victoria Bushman, Jenny Heidbrink, William Fitzhugh, Karen Van Orden, Tao He, Aiqun Li |
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| Rok vydání: | 2012 |
| Předmět: |
Proteomics
Small interfering RNA Integrin beta Chains Cell Survival Endocrinology Diabetes and Metabolism Quantitative proteomics Biology Article Thromboplastin Membrane Cofactor Protein Endocrinology Pancreatic tumor RNA interference Cell Line Tumor Pancreatic cancer Internal Medicine medicine Humans Cell Proliferation Gene knockdown Membrane Glycoproteins Hepatology Reverse Transcriptase Polymerase Chain Reaction Cell growth Membrane Proteins Flow Cytometry medicine.disease Immunohistochemistry Neoplasm Proteins Cell biology Gene Expression Regulation Neoplastic Pancreatic Neoplasms Cancer cell RNA Interference |
| Zdroj: | Pancreas |
| ISSN: | 0885-3177 |
| Popis: | Objectives There is a clear need for better therapeutics and diagnostics for pancreatic cancer. We aimed to discover plasma membrane-associated proteins overexpressed in pancreatic cancer using quantitative proteomics and apply RNA interference (RNAi) to uncover proteins associated with cancer cell survival. Methods Cell surface glycoproteins from 5 pancreatic cancer cell lines were isolated, and differential analyses were performed using mass spectrometry and the "normoid" cell line Hs766T as the comparator. For validation, immunohistochemistry was performed on tissues from 10 independent patients and 2 normal donors. Correlation of protein and mRNA expression level was determined, and functional activity characterized using RNAi. Results Integrin β6, CD46, tissue factor, and a novel protein, chromosome 14 open reading frame 1, were identified as overexpressed on pancreatic cancer cell lines. Immunohistochemistry demonstrated the 4 targets were overexpressed in 20% to 70% of primary pancreatic tumor specimens. Small interfering RNA knockdown resulted in a reduction of cellular proliferation by inhibiting DNA synthesis, blocking S-phase progression or induction of apoptosis. Conclusions By combining a mass spectrometry identification platform and an RNAi validation platform, we have identified a panel of cell surface glycoproteins that not only are overexpressed, but also play a functional role in pancreatic tumor cell survival. |
| Databáze: | OpenAIRE |
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