Monoclonal antibodies against pig transferrin. Blocking and binding activity
| Autor: | I. Trebichavský, V. Viklický, M. Koukal, B. Macků, P. Šiman |
|---|---|
| Rok vydání: | 1987 |
| Předmět: |
medicine.drug_class
Swine Fluorescent Antibody Technique Antigen-Antibody Complex Biology Immunofluorescence Monoclonal antibody Microbiology Sepharose Receptors Transferrin medicine Animals Lymphocytes Receptor Cells Cultured chemistry.chemical_classification Hybridomas medicine.diagnostic_test Cell Membrane Transferrin Antibodies Monoclonal General Medicine Human serum albumin Molecular biology Biochemistry chemistry Animals Newborn Monoclonal biology.protein Swine Miniature Antibody medicine.drug |
| Zdroj: | Folia microbiologica. 32(5) |
| ISSN: | 0015-5632 |
| Popis: | Monoclonal mouse anti-pig transferrin antibodies PTF-01, PTF-02 and PTF-03 and anti-human transferrin antibody HTF-14 detect transferrin coupled with Sepharose particles in an indirect immunofluorescence test. Only the PTF-03 antibody can be used for immunofluorescence detection of pig transferrin bound to specific receptors on the plasma membrane. The binding of iodinated pig transferrin to PK cells was studied. It could be blocked by non-labelled transferrin in excess, by pig serum or by anti-pig transferrin monoclonal antibodies. PTF-03 expressed the lowest blocking activity among the antibodies tested. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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