An Extract from the Plant Deschampsia antarctica Protects Fibroblasts from Senescence Induced by Hydrogen Peroxide

Autor: Ana Jiménez, Francisca Sevilla, Angeles Juarranz, Salvador González, Esther Morel, Ana Ortiz-Espín, Antonio Guerrero
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Oxidative Medicine and Cellular Longevity, Vol 2017 (2017)
Oxidative Medicine and Cellular Longevity
Repositorio Institucional de la Consejería de Sanidad de la Comunidad de Madrid
Consejería de Sanidad de la Comunidad de Madrid
ISSN: 1942-0994
1942-0900
Popis: The Antarctic plantDeschampsia antarctica(DA) is able to survive in extreme conditions thanks to its special mechanism of protection against environmental aggressions. In this work, we investigated whether an aqueous extract of the plant (EDA) retains some of its defensive properties and is able to protect our skin against common external oxidants. We evaluated EDA over young human fibroblasts and exposed to H2O2, and we measured cell proliferation, viability, and senescence-associatedβ-galactosidase (SA-β-Gal). We also tested the expression of several senescence-associated proteins including sirtuin1, lamin A/C, the replicative protein PCNA, and the redox protein thioredoxin 2. We found that EDA promotedper secell proliferation and viability and increased the expression of anti-senescence-related markers. Then, we selected a dose of H2O2as an inductor of senescence in human fibroblasts, and we found that an EDA treatment 24 h prior H2O2exposure increased fibroblast proliferation. EDA significantly inhibited the increase in SA-β-Gal levels induced by H2O2and promoted the expression of sirtuin 1 and lamin A/C proteins. Altogether, these results suggest that EDA protects human fibroblasts from cellular senescence induced by H2O2, pointing to this compound as a potential therapeutic agent to treat or prevent skin senescence.
Databáze: OpenAIRE
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