Improved detection of diffuse large B-cell lymphoma by flow cytometric immunophenotyping-Effect of tissue disaggregation method
| Autor: | C. Tyer, Bethany D. Vallangeon, Beverly Williams, Anand S. Lagoo |
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| Rok vydání: | 2015 |
| Předmět: |
Adult
Male 0301 basic medicine Pathology medicine.medical_specialty Histology Population Biology Immunophenotyping Pathology and Forensic Medicine Flow cytometry 03 medical and health sciences symbols.namesake 0302 clinical medicine medicine Humans education Fisher's exact test Cell Aggregation B-Lymphocytes education.field_of_study medicine.diagnostic_test Cell Biology Middle Aged Flow Cytometry medicine.disease Cell aggregation 030104 developmental biology 030220 oncology & carcinogenesis Tissue and Organ Harvesting symbols Immunohistochemistry Female Lymphoma Large B-Cell Diffuse Diffuse large B-cell lymphoma Cytometry |
| Zdroj: | Cytometry Part B: Clinical Cytometry. 90:455-461 |
| ISSN: | 1552-4949 |
| Popis: | Background Flow cytometric immunophenotyping (FCI) is recognized as a rapid, sensitive, and accurate method for diagnosis of B-cell lymphomas. We observed that FCI failed to identify the clonal B-cell population in several cases of large B-cell lymphoma (DLBCL) when tissue samples were prepared by a commercially available mechanical tissue disaggregation method. We tested a manual tissue disaggregation method and compared it with the mechanical method. Methods FCI findings from 51 cases of DLBCL processed with the mechanical tissue disaggregation method, 27 cases processed using the manual method, and 15 cases processed using a combination of both methods were compared. The histological and immunohistochemical findings in each case were reviewed. Results FCI detected a clonal B-cell population in 88.9% of cases processed by the manual tissue disaggregation method, 66.7% of cases processed by a combination of the manual and mechanical disaggregation methods, and in 62.7% of cases processed solely by the mechanical tissue disaggregation method (P |
| Databáze: | OpenAIRE |
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