Evaluation of Optical Detection Platforms for Multiplexed Detection of Proteins and the Need for Point-of-Care Biosensors for Clinical Use
| Autor: | Kim E. Sapsford, Samantha Spindel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Technology Assessment
Biomedical Computer science Point-of-Care Systems Protein Array Analysis Nanotechnology Biosensing Techniques lcsh:Chemical technology Biochemistry Multiplexing Article Analytical Chemistry Multiplex lcsh:TP1-1185 in vitro diagnostics Electrical and Electronic Engineering Instrumentation Point of care Immunoassay fluorescent quantum dot Optical Devices Equipment Design Microfluidic Analytical Techniques Atomic and Molecular Physics and Optics multiplex Equipment Failure Analysis Computer architecture point-of-care biomarker ELISA protein Biosensor Biological network |
| Zdroj: | Sensors (Basel, Switzerland) Sensors, Vol 14, Iss 12, Pp 22313-22341 (2014) |
| ISSN: | 1424-8220 |
| Popis: | This review investigates optical sensor platforms for protein multiplexing, the ability to analyze multiple analytes simultaneously. Multiplexing is becoming increasingly important for clinical needs because disease and therapeutic response often involve the interplay between a variety of complex biological networks encompassing multiple, rather than single, proteins. Multiplexing is generally achieved through one of two routes, either through spatial separation on a surface (different wells or spots) or with the use of unique identifiers/labels (such as spectral separation—different colored dyes, or unique beads—size or color). The strengths and weaknesses of conventional platforms such as immunoassays and new platforms involving protein arrays and lab-on-a-chip technology, including commercially-available devices, are discussed. Three major public health concerns are identified whereby detecting medically-relevant markers using Point-of-Care (POC) multiplex assays could potentially allow for a more efficient diagnosis and treatment of diseases. |
| Databáze: | OpenAIRE |
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