Transfection of STAT3 overexpression plasmid mediated through recombinant lentivirus promotes differentiation of bone marrow mesenchymal stem cells into neural cells in fetal rats with spina bifida aperta
| Autor: | Mingyu Jiang, Mingyong Ren, Jicheng Dai, Chunming Jiang, Rong Fu, Xu Liu, Yanbo Pan, Yunpeng Hao, Jiale Feng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Male
STAT3 Transcription Factor Aging Enolase Retinoic acid Bone Marrow Cells Tretinoin Biology Mesenchymal Stem Cell Transplantation Transfection Nestin Andrology chemistry.chemical_compound Fetus Western blot medicine Animals nerve cells Rats Wistar signal transducer and activator of transcription-3 Tissue Engineering medicine.diagnostic_test Glial fibrillary acidic protein Lentivirus bone marrow mesenchymal stem cells Cell Differentiation Mesenchymal Stem Cells Cell Biology Rats Transplantation Spina Bifida Cystica lentivirus transfection spina bifida aperta Spinal Cord chemistry biology.protein Female Research Paper Plasmids |
| Zdroj: | Aging (Albany NY) |
| ISSN: | 1945-4589 |
| DOI: | 10.18632/aging.203524 |
| Popis: | We investigated the influence of signal transducer and activator of transcription-3 (STAT3) on the spinal cord tissue grafts of rat fetuses with spina bifida aperta. In particular, we hoped to identify whether transfection of the STAT3 overexpression plasmid increases the survival of spinal cord transplantation in order to improve therapeutic efficacy. The fetal rat model of spina bifida aperta was established using retinoic acid and treated with a microsurgical injection of bone marrow mesenchymal stem cells (BMSCs). The animals were divided into either the blank control group, negative control group or the experimental group. The optical density (OD) value of BMSCs viability was determined using the Cell Counting Kit-8 (CCK-8). The expression of STAT3, phosphorylated STAT3 (pSTAT3), neural markers and apoptosis-related factors were evaluated using real-time PCR and Western blot. The OD value in the experimental group was highest at eight hours after transplantation using CCK-8. The expression of pSTAT3, glial fibrillary acidic protein, neuron-specific enolase, neurofilament and nestin in the experimental group was significantly higher compared to the blank control group and negative control group (P |
| Databáze: | OpenAIRE |
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