Structural and functional evidence that initiation and elongation of HIV-1 reverse transcription are distinct processes
| Autor: | Bernard Ehresmann, Roland Marquet, Jean-Marc Lanchy, Catherine Isel, Chantal Ehresmann |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
biology
Base Sequence Transcription Genetic Molecular Sequence Data RNA General Medicine RNA Transfer Amino Acyl biology.organism_classification Biochemistry Molecular biology Reverse transcriptase HIV Reverse Transcriptase Cell biology Retrovirus Retroviridae Transcription (biology) Transfer RNA biology.protein HIV-1 Humans Nucleic Acid Conformation RNA Viral RNase H Primer binding site Polymerase |
| Zdroj: | Biochimie. 78(11-12) |
| ISSN: | 0300-9084 |
| Popis: | Retroviral reverse transcription starts with the extension of a cellular tRNA primer bound near the 5′ end of the viral genomic RNA at a site called the primer binding site (PBS). Formation of the HIV-1 initiation complex between tRNA3Lys, viral RNA and reverse transcriptase probably occurs during encapsidation of these components. tRNA3Lys is thought to be selectively packaged by interaction with the reverse transcriptase domain of the Pr160Gag-Pol precursor protein, then annealed to the PBS of viral RNA with the help of the nucleocapsid protein. tRNA3Lys and HIV-1 viral RNA form a highly-structured complex, with extended interactions between the two molecules. Two different modes of reverse transcription have been distinguished: initiation, a tRNA3Lys-specific and distributive mode of polymerization corresponding to the addition of the first five nucleotides, followed by elongation, a non-specific and processive mode of DNA synthesis. These two modes are reminiscent of the initiation and elongation processes previously observed with DNA-dependent RNA polymerases. |
| Databáze: | OpenAIRE |
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