First report of V1016G and S989P knockdown resistant (kdr) mutations in pyrethroid-resistant Sri Lankan Aedes aegypti mosquitoes
| Autor: | Nissanka K de Silva, William C. Black, Sachini D. Fernando, Rushika Perera, Karla Saavedra-Rodriguez, Menaka Hapugoda |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Insecticides Pyrethroid resistance Genotype 030231 tropical medicine Short Report Mosquito Vectors Aedes aegypti law.invention Dengue fever lcsh:Infectious and parasitic diseases Insecticide Resistance 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Aedes law Pyrethrins parasitic diseases medicine Voltage-gated sodium channel Animals lcsh:RC109-216 Alleles Polymerase chain reaction Sri Lanka Pyrethroid biology Knockdown resistance biology.organism_classification medicine.disease Virology 030104 developmental biology Infectious Diseases chemistry Parasitology Vector (epidemiology) kdr Mutation Insect Proteins |
| Zdroj: | Parasites & Vectors, Vol 11, Iss 1, Pp 1-6 (2018) Parasites & Vectors |
| ISSN: | 1756-3305 |
| DOI: | 10.1186/s13071-018-3113-0 |
| Popis: | Background Dengue is a serious arboviral disease in Sri Lanka with a large number of dengue fever (DF) cases every year. Control of the primary vector Aedes aegypti depends upon larval habitat source reduction and insecticide application. However, increases in the number of reported cases suggest the inefficiency of current control strategies and the possibility of resistance to currently used insecticides. Early detection of mutations in the voltage-gated sodium channel (vgsc) gene that confer knockdown resistance (kdr) to pyrethroid insecticides is important in resistance management in vector populations. Results Resistance to pyrethroid insecticides was detected in the three populations studied. Polymerase chain reaction was used to detect the presence of two kdr mutations F1534C and V1016G. During this process a S989P mutation was also detected in pyrethroid-resistant Ae. aegypti populations. These mutations were found to be widespread and frequent in the collections studied. Conclusions To our knowledge, this study reveals for the first time the presence of V1016G and S989P mutant alleles in the vgsc of Sri Lankan Ae. aegypti populations. The spread of the mutant alleles throughout the country poses a threat of increased resistance to pyrethroids. Long-term insecticide applications and indiscriminate use of pyrethroids has led to the evolution of resistance. More strategic and diverse strategies, including novel insecticides with new modes of action and community participation, should be engaged for Ae. aegypti control. Electronic supplementary material The online version of this article (10.1186/s13071-018-3113-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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