Improving endothelial healing with novel chimeric mitogens
Autor: | Luke Brewster, Vicki A. Husak, Lian Xue, Joan Ellinger, Eric M. Brey, Howard P. Greisler, Michael Addis, Christian C. Haudenschild |
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Rok vydání: | 2006 |
Předmět: |
medicine.medical_specialty
Mitotic index medicine.medical_treatment Recombinant Fusion Proteins Mutant Chimeric Proteins Pharmacology Fibroblast growth factor Dogs Angioplasty Internal medicine Mitotic Index Medicine Animals Fibroblast Fibrin glue Receptor Growth Substances Wound Healing business.industry General Medicine Endothelial stem cell medicine.anatomical_structure Endocrinology Models Animal Microscopy Electron Scanning Cytokines Fibroblast Growth Factor 1 Surgery Endothelium Vascular business Carotid Artery Injuries Carrier Proteins Tunica Intima Tunica Media Blood vessel |
Zdroj: | American journal of surgery. 192(5) |
ISSN: | 1879-1883 |
Popis: | Background Chimeric proteins may be used to direct cell-specific activity. Heparin-binding growth-associated molecule (HBGAM) binds to cell receptors that are relatively more robust on endothelial cells, and it may confer endothelial cell selectivity to potent angiogens such as fibroblast growth factor-1 (FGF-1). Methods By ligating fibroblast growth factor or its potent mutant, S130K, to HBGAM, we tested their effect on re-endothelialization after angioplasty injury by using a canine model. Results Both HBGAM/S130K- and HBGAM/FGF-1–treated arteries had increased neointimal mitotic index and re-endothelialization rates at 30 days compared with control arteries without inducing a significant increase in the neointimal thickness or the ratio of neointimal to medial thickness between treatment and control groups. Conclusion HBGAM/S130K and HBGAM/FGF-1 facilitates endothelial healing without myointimal thickening after canine carotid artery balloon angioplasty injury. Application of these growth factors in fibrin glue may improve endothelialization clinically after angioplasty or endarterectomy. |
Databáze: | OpenAIRE |
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