The Nonsense-Mediated mRNA Decay pathway degrades dendritically-targeted mRNAs to regulate long-term potentiation and cognitive function
| Autor: | Megan Allen, Francesco Longo, Dilek Colak, Miklós Tóth, Michael Notaras, Nicole Volk, Li Jeon N, Eric Klann |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0303 health sciences
media_common.quotation_subject Nonsense-mediated decay RNA Long-term potentiation Translation (biology) Biology Cell biology 03 medical and health sciences 0302 clinical medicine Synaptic plasticity Protein biosynthesis Regulatory Pathway Internalization 030217 neurology & neurosurgery 030304 developmental biology media_common |
| DOI: | 10.1101/389585 |
| Popis: | Synaptic plasticity relies on new protein synthesis in dendrites that involves the selective translation of specific mRNAs. This requires a tight control of mRNA levels in dendrites. Consistently, RNA translation and degradation pathways have been recently linked to neurodevelopmental and neuropsychiatric diseases, suggesting a role for RNA regulation in synaptic plasticity and cognition. Despite being the only RNA regulatory pathway that is associated with multiple mental illnesses, the Nonsense-Mediated mRNA Decay (NMD) pathway presents an unexplored regulatory mechanism for synaptic function and plasticity. NMD is a highly conserved and selective RNA degradation pathway that exerts its function in a cell- and spatiotemporally-specific manner. Here, we show that neuron-specific disruption of NMD in adulthood attenuates learning, memory, hippocampal LTP, and potentiates perseverative/repetitive behavior. While it is known that local translation of specific mRNAs in dendrites enables synaptic plasticity, the tightly-controlled mechanisms that regulate local quantity of specific mRNAs remains poorly understood. We report that the NMD pathway operates within dendrites to regulate GluR1 surface levels. Specifically, NMD modulates the internalization of GluR1 and promotes its local synthesis in dendrites. We identified AMPK as a mechanistic substrate for NMD that contributes to the NMD-mediated regulation of GluR1 by limiting total GluR1 levels. These data establish that NMD regulates synaptic plasticity, cognition, and local protein synthesis in dendrites, providing fundamental insight into the neuron-specific function of NMD within the brain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|