Cytotoxic activity of 13-hydroxyoctadecadienoic acid againstToxoplasma gondii
| Autor: | E. Y. Chi, W. R. Henderson |
|---|---|
| Rok vydání: | 1992 |
| Předmět: |
biology
Linoleic acid 13-Hydroxyoctadecadienoic acid Hydroxyeicosatetraenoic acid Toxoplasma gondii biology.organism_classification Antithrombins Microbiology Linoleic Acid Microscopy Electron chemistry.chemical_compound Thromboxane A2 Infectious Diseases Linoleic Acids chemistry Eicosanoid Hydroxyeicosatetraenoic Acids Animals Cytotoxic T cell Animal Science and Zoology Parasitology Arachidonic acid 12-Hydroxy-5 8 10 14-eicosatetraenoic Acid Toxoplasma |
| Zdroj: | Parasitology. 105:343-347 |
| ISSN: | 1469-8161 0031-1820 |
| Popis: | SUMMARYRecent data indicate that platelets may play an important role in the host defence againstToxoplasma gondiiinfections.T. gondii-stimulated human platelets release thromboxane A2(TXA2) and 12-hydroxyeicosatetraenoic acid (12-HETE) from arachidonic acid and 13-hydroxyoctadecadienoic acid (13-HODE) from linoleic acid (Yonget al.1991; Hendersonet al.1992). We have previously demonstrated that the eicosanoid TXA2has potent cytotoxic activity againstT. gondiitrophozoites (Yonget al.1991). In this study, we examined whether 12-HETE, 13-HODE, and linoleic acid also have toxoplasmacidal activity. 13-HODE at concentrations ≥ 10−8M rapidly induced cytotoxic changes inT. gondii. Ultrastructural changes induced by 13-HODE inT. gondiiincluded an initial leakage of cytoplasmic contents into a space between the inner and outer parasite bilayer membrane units which was followed by intracellular vacuolation and loss of cytoplasmic contents. In contrast, linoleic acid and 12-HETE lacked toxoplasmacidal activity at 10−10–10−6M concentrations. These data indicate that 13-HODE, a product of linoleic acid metabolism, has potent cytotoxic activity againstT. gondii; this toxoplasmacidal activity may be important in the inflammatory response to this pathogen. |
| Databáze: | OpenAIRE |
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