Phenotypic characterization of diamond (dind), a Drosophila gene required for multiple aspects of cell division
| Autor: | Francesca Cipressa, Michael L. Goldberg, Maurizio Gatti, Silvia Bonaccorsi, Giovanni Cenci, Lucia Graziadio, Byron C. Williams, Valeria Palumbo |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Male Centriole Green Fluorescent Proteins Mitosis Cell Cycle Proteins Biology Chromosome segregation Animals Genetically Modified 03 medical and health sciences 0302 clinical medicine Male meiosis Meiosis Spermatocytes Chromosome Segregation Genetics Endoreduplication Animals Drosophila Proteins Chromosome aberrations Genetics (clinical) chromosome condensation Diamond gene Centriole fragmentation Brain Chromosome Breakage Chromatin Cell biology Chromosomes Insect 030104 developmental biology Phenotype Centrosome Premature chromosome condensation Larva Mutation Drosophila 030217 neurology & neurosurgery Cell Division Diamond gene Mitosis Male meiosis chromosome condensation Chromosome aberrations Chromosome segregation Endoreduplication Centriole fragmentation Drosophila |
| Zdroj: | Chromosoma (Berl., Print) 127 (2018): 489–504. doi:10.1007/s00412-018-0680-y info:cnr-pdr/source/autori:Graziadio L.; Palumbo V.; Cipressa F.; Williams B.C.; Cenci G.; Gatti M.; Goldberg M.L.; Bonaccorsi S./titolo:Phenotypic characterization of diamond (dind), a Drosophila gene required for multiple aspects of cell division/doi:10.1007%2Fs00412-018-0680-y/rivista:Chromosoma (Berl., Print)/anno:2018/pagina_da:489/pagina_a:504/intervallo_pagine:489–504/volume:127 |
| ISSN: | 1432-0886 |
| Popis: | Many genes are required for the assembly of the mitotic apparatus and for proper chromosome behavior during mitosis and meiosis. A fruitful approach to elucidate the mechanisms underlying cell division is the accurate phenotypic characterization of mutations in these genes. Here, we report the identification and characterization of diamond (dind), an essential Drosophila gene required both for mitosis of larval brain cells and for male meiosis. Larvae homozygous for any of the five EMS-induced mutations die in the third-instar stage and exhibit multiple mitotic defects. Mutant brain cells exhibit poorly condensed chromosomes and frequent chromosome breaks and rearrangements; they also show centriole fragmentation, disorganized mitotic spindles, defective chromosome segregation, endoreduplicated metaphases, and hyperploid and polyploid cells. Comparable phenotypes occur in mutant spermatogonia and spermatocytes. The dind gene encodes a non-conserved protein with no known functional motifs. Although the Dind protein exhibits a rather diffuse localization in both interphase and mitotic cells, fractionation experiments indicate that some Dind is tightly associated with the chromatin. Collectively, these results suggest that loss of Dind affects chromatin organization leading to defects in chromosome condensation and integrity, which in turn affect centriole stability and spindle assembly. However, our results do not exclude the possibility that Dind directly affects some behaviors of the spindle and centrosomes. |
| Databáze: | OpenAIRE |
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