Designer self-assembling peptide scaffold stimulates pre-osteoblast attachment, spreading and proliferation
| Autor: | Zhi-Jian Xie, Fei-Qing Hu, Ling-Fei Ren, Feng Zhang, Geng-Sheng Shi, Sheng-lai Li |
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| Rok vydání: | 2008 |
| Předmět: |
Scaffold
Materials science Cell Biomedical Engineering Biophysics Cell Culture Techniques Bioengineering Peptide Cell Line Biomaterials Mice Cell Movement Osteogenesis Fluorescence microscope medicine Cell Adhesion Animals Cells Cultured Cell Proliferation chemistry.chemical_classification Osteoblasts Tissue Engineering Cell growth Osteoblast Extracellular Matrix medicine.anatomical_structure Biochemistry chemistry Cell culture Drug Design Multiprotein Complexes Peptides Self-assembling peptide |
| Zdroj: | Journal of materials science. Materials in medicine. 20(7) |
| ISSN: | 1573-4838 |
| Popis: | A new peptide scaffold was made by mixing pure RADA16 (Ac-RADARADARADARADA-CONH2) and designer peptide RGDA16 (Ac-RADARGDARADARGDA-CONH2) solutions, and investigate any effect on attachment, spreading and proliferation of pre-osteoblast (MC3T3-E1). The peptides, RADA16 and RGDA16, were custom-synthesized. They were solubilized in deionized water at a concentration of 10 mg/ml (1% w/v), the RGDA16 peptide solution was mixed 1:1 with RADA16 solution and a new peptide solution RGDAmix was produced. The RGDAmix and RADA16 solution were directly loaded in 96-well plates and cover slips, and two different peptide scaffolds were formed with the addition of maintenance medium (alpha-MEM) in several minutes. About 1.0 x 10(4) MC3T3-E1 cells were seeded on each hydrogel scaffold, and then the cell morphological changes were observed using a fluorescence microscope at 1 h, 3 h and 24 h timepoint, respectively. Cell attachment was evaluated 1 h, 3 h and 24 h after cell seeding and cell proliferation was determined 4d, 7d and 14d after cell seeding. The RGDAmix scaffold significantly promoted the initial cell attachment compared with the RADA16 scaffold. MC3T3-E1 cells adhered and spread well on both scaffolds, however, cells spread better on the RGDAmix scaffold than on the RADA16 scaffold. Cell proliferation was greatly stimulated when cultured on RGDAmix scaffold. The RGD sequence contained peptide scaffold RGDAmix significantly enhances MC3T3-E1 cells attachment, spreading and proliferation. |
| Databáze: | OpenAIRE |
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