Climbing the vertebrate branch of U1A/U2B″ protein evolution
| Autor: | Sandra G. Williams, Kimberly Delaney, Mariah N Lawler, Kathleen B. Hall |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Ancestral reconstruction
Models Molecular Protein family Genetic Speciation Lineage (evolution) genetic processes Molecular Sequence Data Biology environment and public health snRNP Core Proteins Ribonucleoprotein U1 Small Nuclear Evolution Molecular Animals Humans snRNP Amino Acid Sequence Molecular Biology Phylogeny Genetics SnRNP Core Proteins Base Sequence Sequence Homology Amino Acid urogenital system fungi RNA Articles Ribonucleoprotein U2 Small Nuclear Vertebrates health occupations Subfunctionalization Small nuclear RNA |
| Popis: | In the vertebrate lineage of the U1A/U2B″/SNF protein family, the U1A and U2B″ proteins bind to RNA stem–loops in the U1 or U2 snRNPs, respectively. However, their specialization is fairly recent, as they evolved from a single ancestral protein. The progress of their specialization (subfunctionalization) can be monitored by the amino acid sequence changes that give rise to their modern RNA-binding specificity. Using ancestral sequence reconstruction to predict the intermediates on the evolutionary branch, a probable path of sequential changes is defined for U1A and U2B″. The RNA-binding affinity for U1A/U2B″ protein ancestors was measured using modern U1 and U2 snRNA stem–loops and RNA stem–loop variants to understand how the proteins’ RNA specificities evolved. |
| Databáze: | OpenAIRE |
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