The thin-layer agar method for direct phenotypic detection of multi- and extensively drug-resistant tuberculosis
| Autor: | L. Goginashvili, B. C. de Jong, H. Pangtey, R. Aspindzelashvili, Wim Mulders, Elisa Ardizzoni, Mathieu Bastard, Francis Varaine, T. Kotrikadze, Leen Rigouts |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Pulmonary and Respiratory Medicine
Ofloxacin Pathology medicine.medical_specialty Veterinary medicine Tuberculosis food.ingredient Antitubercular Agents Microbial Sensitivity Tests Drug resistance Sensitivity and Specificity Mycobacterium tuberculosis food Kanamycin Drug Resistance Multiple Bacterial Isoniazid medicine Humans Agar biology business.industry Sputum Extensively drug-resistant tuberculosis medicine.disease biology.organism_classification bacterial infections and mycoses Infectious Diseases Human medicine Rifampin business Rifampicin Fluoroquinolones medicine.drug |
| Zdroj: | International journal of tuberculosis and lung disease |
| ISSN: | 1027-3719 |
| Popis: | SUMMARY BACKGROUND: Molecular techniques rapidly detect resistance to rifampicin (RMP) and isoniazid (INH), but do not eliminate the need for culture-based drug susceptibility testing (DST) against other drugs. The thin-layer agar (TLA) test, a non-commercial direct DST method, has demonstrated good performance for INH and RMP; however, evidence is still limited, and its applicability for DST of ofloxacin (OFX) and kanamycin (KM) is unknown. DESIGN: We compared 279 TLA DST results with those of MGIT for INH and RMP, and 280 results for OFX and KM with those of the 7H11 agar proportion method, obtained from 320 smear-positive samples from 165 Georgian TB patients. Discrepancies were solved by comparison with a composite reference standard. The prevalence of multidrug-resistant tuberculosis (TB) was 30 of 164 patients (18.3%), 2 (6.7%) of whom had extensively drug-resistant TB. RESULTS: TLA showed 94.7%, 98.2%, 100% and 78.9% sensitivity, respectively, for INH, RMP, OFX and KM, with 100% specificity. Average time to results was 7 days in TLA, 23 in MGIT and 49 for 7H11 agar. CONCLUSIONS: In low-resource settings, TLA can be applied for the rapid detection of resistance to INH, RMP and fluoroquinolones. Further studies are necessary to improve sensitivity to KM and further assess its performance for OFX and other drugs and its applicability in field conditions. KEY W ORDS: resistance detection; non-commercial method; tuberculosis; MDR-TB; XDR-TB |
| Databáze: | OpenAIRE |
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