High molecular prevalence of Leishmania in phlebotomine sand flies fed on chicken blood in Brazil
| Autor: | Thaís Tâmara Castro Minuzzi-Souza, Tauana de Sousa Ferreira, Nadjar Nitz, Douglas de Almeida Rocha, Mariana Neiva, Mariana Hecht, Joana de Albuquerque Ribeiro, Renata Velozo Timbó, Paulo Silva de Almeida, Rodrigo Gurgel-Gonçalves |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Wet season Veterinary medicine Mitochondrial DNA Endemic Diseases 030231 tropical medicine Lutzomyia longipalpis Polymerase Chain Reaction 03 medical and health sciences 0302 clinical medicine parasitic diseases Prevalence medicine Animals Humans Transition Temperature Leishmania infantum Leishmaniasis Meals General Veterinary biology DNA Kinetoplast Feeding Behavior Sequence Analysis DNA General Medicine biology.organism_classification Leishmania medicine.disease Insect Vectors Sandfly Blood 030104 developmental biology Vector (epidemiology) Female Parasitology Seasons Psychodidae Chickens Brazil |
| Zdroj: | Veterinary Parasitology. 259:80-84 |
| ISSN: | 0304-4017 |
| DOI: | 10.1016/j.vetpar.2018.07.004 |
| Popis: | Leishmaniases are endemic in Brazil, where Leishmania infantum has been detected in humans, dogs, cats, and phlebotomine vectors. Monitoring synanthropic vector populations is critical for leishmaniasis control-surveillance in such transmission-prone areas. Here, a suite of molecular approaches were used to assess Leishmania infection prevalence and to identify blood-meal sources in a large sample of sand flies collected in anthropic environments of a Leishmania-transmission area in Mato Grosso do Sul State (Rio Verde de Mato Grosso municipality), Central-West Brazil. We sampled sand flies monthly (January-June 2014 and 2016) in one peri-domestic site within each of six neighborhoods with recent records of human visceral and/or tegumentary leishmaniasis. kDNA-qPCR plus rDNA ITS-sequencing were used to detect and identify Leishmania in pooled female sand flies. Individual engorged females (n = 58) were used for blood-meal analyses through High-Resolution Melting (HRM) targeting the mtDNA cytb gene. Overall, 90.5% of 420 CDC trap-nights yielded vectors, for a total catch of 24,989 sand flies. We sub-sampled and identified 3088 sand flies of 12 species, including 2775 Lutzomyia longipalpis (the most abundant species at all sampling sites) and 297 Nyssomyia whitmani. Female sand flies (n = 1261) were grouped in 159 pools, of which 92 Lu. longipalpis (minimum infection rate [MIR] 8%) and 7 Ny. whitmani pools (MIR 7%) were Leishmania kDNA-positive. Most positive Lu. longipalpis were collected in the 2016 rainy season. Sequencing confirmed L. infantum in Lu. longipalpis samples. HRM analyses identified chicken DNA in 57 sand flies (98.3%), 37 of which were Leishmania DNA-positive (64.9%); human blood was found in just one (Leishmania-negative) female. Our data show ongoing risk of L. infantum transmission to humans in the study area, where Leishmania-infected sandfly vectors are common and heavily rely on chicken blood in the peri-domestic environment. |
| Databáze: | OpenAIRE |
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