Genetic subtraction profiling identifies genes essential for Arabidopsis reproduction and reveals interaction between the female ganetophyte and the maternal sporophyte
| Autor: | Samuel Ej Wuest, Michael Federer, Ueli Grossniklaus, Jacqueline Gheyselinck, Jörg Becker, Patrick Meier, Edith Schlagenhauf, Amal J. Johnston |
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| Jazyk: | angličtina |
| Rok vydání: | 2007 |
| Předmět: |
0106 biological sciences
Egg cell animal structures Mutant Arabidopsis Extraembryonic Membranes Biology Genes Plant 01 natural sciences embryology/genetics Genes Plant/genetics Transcriptome 03 medical and health sciences medicine Polycomb Group Gene Arabidopsis/genetics Ovule Gene In Situ Hybridization 030304 developmental biology Gametophyte Genetics 0303 health sciences Gene Expression Profiling Reproduction Research Genes Homeobox Gene Expression Regulation Developmental Embryo Independent Seed Development Microarray Analysis Gene expression profiling medicine.anatomical_structure embryonic structures Transcription Factors 010606 plant biology & botany |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal Repositório Científico de Acesso Aberto de Portugal (RCAAP) instacron:RCAAP Genome Biology |
| Popis: | Genetic subtraction and expression profiling of wild-type Arabidopsis and a sporophytic mutant lacking an embryo sac identified 1,260 genes expressed in the embryo sac; a total of 527 genes were identified for their expression in ovules of mutants lacking an embryo sac. Background The embryo sac contains the haploid maternal cell types necessary for double fertilization and subsequent seed development in plants. Large-scale identification of genes expressed in the embryo sac remains cumbersome because of its inherent microscopic and inaccessible nature. We used genetic subtraction and comparative profiling by microarray between the Arabidopsis thaliana wild-type and a sporophytic mutant lacking an embryo sac in order to identify embryo sac expressed genes in this model organism. The influences of the embryo sac on the surrounding sporophytic tissues were previously thought to be negligible or nonexistent; we investigated the extent of these interactions by transcriptome analysis. Results We identified 1,260 genes as embryo sac expressed by analyzing both our dataset and a recently reported dataset, obtained by a similar approach, using three statistical procedures. Spatial expression of nine genes (for instance a central cell expressed trithorax-like gene, an egg cell expressed gene encoding a kinase, and a synergid expressed gene encoding a permease) validated our approach. We analyzed mutants in five of the newly identified genes that exhibited developmental anomalies during reproductive development. A total of 527 genes were identified for their expression in ovules of mutants lacking an embryo sac, at levels that were twofold higher than in the wild type. Conclusion Identification of embryo sac expressed genes establishes a basis for the functional dissection of embryo sac development and function. Sporophytic gain of expression in mutants lacking an embryo sac suggests that a substantial portion of the sporophytic transcriptome involved in carpel and ovule development is, unexpectedly, under the indirect influence of the embryo sac. |
| Databáze: | OpenAIRE |
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