The HMG Box Transcription Factor Sox4 Contributes to the Development of the Endocrine Pancreas
| Autor: | Yasuhiro Kosaka, Anna Kalousova, Hans Clevers, Michael S. German, Janet Lau, Maria E. Wilson, Juehu Wang, Caroline Mrejen, Katherine Yang, Vasso Episkopou, Francis C. Lynn |
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| Rok vydání: | 2005 |
| Předmět: |
Male
endocrine system medicine.medical_specialty Endocrinology Diabetes and Metabolism Enteroendocrine cell Biology SOXC Transcription Factors SOX Transcription Factors Islets of Langerhans Mice SOX2 Internal medicine Ectoderm Internal Medicine medicine Animals Pancreas DNA Primers Mice Knockout Reverse Transcriptase Polymerase Chain Reaction Pancreatic islets High Mobility Group Proteins Gene Expression Regulation Developmental Nuclear Proteins Sex-Determining Region Y Protein Cell biology DNA-Binding Proteins medicine.anatomical_structure Testis determining factor Endocrinology HMG-Box Domains Trans-Activators Pancreatic bud formation Stem cell SOXD Transcription Factors |
| Zdroj: | Diabetes. 54:3402-3409 |
| ISSN: | 1939-327X 0012-1797 |
| DOI: | 10.2337/diabetes.54.12.3402 |
| Popis: | To investigate the role of the Sry/hydroxymethylglutaryl box (Sox) transcription factors in the development of the pancreas, we determined the expression pattern of Sox factors in the developing mouse pancreas. By RT-PCR, we detected the presence of multiple Sox family members in both the developing pancreas and mature islets and then focused on two factors, Sox2 and Sox4. The expression field of Sox2, which plays a role in the maintenance of some stem cell populations, included the developing duodenum, but Sox2 was specifically excluded from the pancreatic buds. In contrast, Sox4 was detected broadly in the early pancreatic buds and eventually became restricted to the nuclei of all islet cells in the adult mouse. Mice homozygous for a null mutation of the sox4 gene showed normal pancreatic bud formation and endocrine cell differentiation up to embryonic day 12.5. Beyond that date, cultured pancreatic explants lacking sox4 failed to form normal islets. Instead, a markedly reduced number of endocrine cells were found scattered through the explant. We show here that several Sox transcription factors are expressed in the developing pancreas and in the islet, and that one of these factors, Sox4, is required for the normal development of pancreatic islets. |
| Databáze: | OpenAIRE |
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