Large specimen bone embedment and cement line staining
| Autor: | Helen E. Gruber, Audrey A. Stasky |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Pathology
medicine.medical_specialty Silver Staining Histology Plastic Embedding medicine.medical_treatment Bone canaliculus Stain Bone and Bones Silver stain Dogs medicine Animals biology Embedment Chemistry Acid phosphatase General Medicine Tetracycline Staining Medical Laboratory Technology medicine.anatomical_structure Spinal fusion biology.protein Methacrylates Cortical bone |
| Zdroj: | Biotechnichistochemistry : official publication of the Biological Stain Commission. 72(4) |
| ISSN: | 1052-0295 |
| Popis: | Undecalcified embedment of large bone specimens is often challenging. A method is presented here that is suitable for methacrylate embedment of sections of canine vertebrae while retaining the ability to localize tartrate-resistant acid phosphatase and alkaline phosphatase activity. Specimens also retained tetracycline labelling, and sectioned preparations were readily stained with routine bone procedures. A modification of the Bodian silver stain, used for examining the nerves and spinal cord in these specimens, provided a useful stain for canaliculi and cement lines in trabecular and cortical bone. This stain is advantageous when both bone and nerve tissue are of interest, as in spinal fusion studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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