Induction of shoot regeneration in cotyledon explants of the oilseed crop Sesamum indicum L
| Autor: | N. Arumugam, Masochon Zimik |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sucrose food.ingredient lcsh:QH426-470 Plant tissue culture lcsh:Biotechnology 01 natural sciences Sesamum Shoot regeneration 03 medical and health sciences Basal shoot Tissue culture Murashige and Skoog medium food lcsh:TP248.13-248.65 Botany General Materials Science biology fungi food and beverages biology.organism_classification lcsh:Genetics 030104 developmental biology Shoot Recalcitrance Cotyledon 010606 plant biology & botany Explant culture |
| Zdroj: | Journal of Genetic Engineering and Biotechnology, Vol 15, Iss 2, Pp 303-308 (2017) |
| Popis: | Sesamum indicum is an ancient oilseed crop known for its high quality edible oil and its medicinally important lignans. The crop is said to be recalcitrant to plant tissue culture thus limiting the use of modern biotechnology for its genetic improvement. We present here a protocol describing plant regeneration through adventitious shoot formation from cotyledons dissected from sesame seeds soaked for four hours in water. Subculturing of the cotyledons after two weeks of culture on to a fresh Murashige and Skoog medium leads to differentiation of adventitious shoots from the proximal cut end of the explant. Culture of cotyledons on a medium containing 9% sucrose for a couple of weeks prior to transfer to MS medium supplemented with 3% sucrose induced a higher frequency of shoot regeneration. The highest frequency of 25% adventitious shoot regeneration was observed for S. indicum variety UMA. This variety also turned out to be the best among the ten genotypes tested for shoot regeneration through tissue culture. While addition of IAA marginally improved regeneration, silver nitrate was found essential for enhancing the frequency of shoot regeneration. The regenerated shoots formed roots on full strength MS medium supplemented with 1 mg/l IBA and the rooted plants were established in soil. |
| Databáze: | OpenAIRE |
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