Chlorogenic acid improves the quality of boar semen processed in Percoll
| Autor: | S. S. Rabelo, B. R. Chaves, W. E. Silva, Luciano José Pereira, Márcio Gilberto Zangeronimo, Carla Oliveira Resende, Juliano Vogas Peixoto, B. A. Pereira, T. P. Pontelo |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
endocrine system
General Veterinary urogenital system Vitamin E medicine.medical_treatment education Extender Semen Insemination Sperm law.invention Andrology reproduction chemistry.chemical_compound antioxidants Chlorogenic acid chemistry law parasitic diseases medicine Animal Science and Zoology Original Article sperm selection Acrosome Percoll |
| Zdroj: | Animal Reproduction |
| ISSN: | 1984-3143 1806-9614 |
| Popis: | This study aimed to evaluate if the addition of chlorogenic acid (ChA) to semen extenders improves the quality of cooled boar semen processed in Percoll. The experimental design was randomized blocks (ejaculates) in a 2×3 factorial (with or without Percoll, and three antioxidant systems: a negative control, without supplementation, a positive control – vitamin E, and ChA), totaling six treatments and 12 repetitions. ChA and vitamin E (VE) were added at 4.5 mg/ml and 400 μg/ml in extender, respectively. At 0, 48 and 72h of storage at 15ºC, 80 ml insemination doses each containing 2.0 billion sperm cells were submitted to centrifugation in Percoll. The use of Percoll impaired (P0.05) in semen stored for 72h. ChA improved (P0.05) when the semen was processed in Percoll. The antioxidants had no effect (P>0.05) on the viability and integrity of the acrosome, but ChA reduced (P0.05) of antioxidants or Percoll on the concentration of malondialdehyde in seminal plasma. The use of Percoll had no effect (P>0.05) on the cholesterol efflux, but ChA increased (P |
| Databáze: | OpenAIRE |
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