Physiological levels of folic acid reveal purine alterations in Lesch-Nyhan disease
| Autor: | Esther L Outtrim, Rong Fu, Hyder A. Jinnah, Diane J. Sutcliffe, Rosa J. Torres, José M. López |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Purine
medicine.medical_specialty Hypoxanthine Phosphoribosyltransferase Lesch-Nyhan Syndrome Cell Culture Techniques chemistry.chemical_compound Cerebrospinal fluid Adenosine Triphosphate Folic Acid Internal medicine medicine Humans Nucleotide Hyperuricemia Purine metabolism chemistry.chemical_classification Multidisciplinary biology business.industry Fibroblasts Ribonucleotides Biological Sciences medicine.disease Aminoimidazole Carboxamide Endocrinology chemistry Hypoxanthine-guanine phosphoribosyltransferase Purines Culture Media Conditioned biology.protein Phosphoribosyltransferase Uric acid business |
| Zdroj: | Proc Natl Acad Sci U S A |
| ISSN: | 1091-6490 |
| Popis: | Lesch-Nyhan disease (LND), caused by a deficient salvage purine pathway, is characterized by severe neurological manifestations and uric acid overproduction. However, uric acid is not responsible for brain dysfunction, and it has been suggested that purine nucleotide depletion, or accumulation of other toxic purine intermediates, could be more relevant. Here we show that purine alterations in LND fibroblasts depend on the level of folic acid in the culture media. Thus, physiological levels of folic acid induce accumulation of 5-aminoimidazole-4-carboxamide riboside 5'-monophosphate (ZMP), an intermediary of de novo purine biosynthetic pathway, and depletion of ATP. Additionally, Z-nucleotide derivatives (AICAr, AICA) are detected at high levels in the urine of patients with LND and its variants (hypoxanthine-guanine phosphoribosyltransferase [HGprt]-related neurological dysfunction and HGprt-related hyperuricemia), and the ratio of AICAr/AICA is significantly increased in patients with neurological problems (LND and HGprt-related neurological dysfunction). Moreover, AICAr is present in the cerebrospinal fluid of patients with LND, but not in control individuals. We hypothesize that purine alterations detected in LND fibroblasts may also occur in the brain of patients with LND. |
| Databáze: | OpenAIRE |
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