Molecular assay for the detection of Cochlosoma anatis in house flies and turkey specimens by polymerase chain reaction
| Autor: | Tanja McKay, Sheri M. McElroy, Allen L. Szalanski, Carrie B. Owens, A. J. Bermudez, C. Dayton Steelman |
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| Rok vydání: | 2005 |
| Předmět: |
House fly
Turkeys Mitochondrial DNA DNA Mitochondrial DNA Ribosomal Polymerase Chain Reaction Article DNA sequencing Microbiology law.invention Feces PEMS law Houseflies RNA Ribosomal 16S Molecular diagnostics Animals Protozoan Infections Animal Poultry Diseases Anatis Polymerase chain reaction DNA Primers Base Sequence General Veterinary biology Cochlosoma anatis fungi General Medicine DNA Protozoan Ribosomal RNA Amplicon biology.organism_classification Molecular biology Insect Vectors Intestines Trichomonadida PCR Female Parasitology Musca |
| Zdroj: | Veterinary Parasitology |
| ISSN: | 0304-4017 |
| Popis: | A 1520 bp region of Cochlosoma anatis mtDNA 16S gene was subjected to DNA sequencing and a 466 bp portion was compared with other protozoan 16S sequences to develop PCR primers specific for C. anatis. This PCR diagnostic method allowed identification of C. anatis from house flies, Musca domestica L., turkey gut, and fecal samples within 6 h after field-collected samples reached the laboratory. House flies detected carrying C. anatis using the diagnostic 374 bp amplicons represented the first record of this protozoan in house flies. |
| Databáze: | OpenAIRE |
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