Functional changes in rat liver mitochondria on administration of 2-methyl-4-dimethylaminoazobenzene
| Autor: | Pothana Saikumar, C K R Kurup |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1984 |
| Předmět: |
Male
medicine.medical_specialty Ubiquinone Submitochondrial Particles Mitochondria Liver Biology Mitochondrion Biochemistry Oxidative Phosphorylation p-Dimethylaminoazobenzene Oral administration Internal medicine Organelle medicine Cytochrome c oxidase Animals Molecular Biology Methyldimethylaminoazobenzene 4-Dimethylaminoazobenzene Proteins Cell Biology Rats Endocrinology Cholesterol Toxicity biology.protein Cytochromes NAD+ kinase Cytochrome aa3 Research Article |
| Zdroj: | IndraStra Global. |
| ISSN: | 2381-3652 |
| Popis: | Administration of 2-methyl-4-dimethylaminobenzene in the diet (0.1%, w/w) for 85-90 days doubled the content of mitochondria in the livers of rats. The azodye was covalently bound to liver proteins, and about 15% of the amount found in liver was associated with the mitochondrial fraction. Mitochondria isolated from the livers of azodye-fed animals showed drastically lowered ability to oxidize NAD+-linked substrates. The inhibited electron-transfer step was the reduction of ubiquinone. The organelles showed a large increase in succinate oxidase activity. The activity of cytochrome oxidase and the content of cytochrome aa3 were substantially higher in these organelles. Azodye-fed animals showed depressed serum cholesterol concentrations. The content of ubiquinone in liver also registered a small increase. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|