Regulation of monocyte cell fate by blood vessels mediated by Notch signalling
| Autor: | Jaba Gamrekelashvili, Roberto Giagnorio, Jasmin Jussofie, Oliver Soehnlein, Johan Duchene, Carlos G. Briseño, Saravana K. Ramasamy, Kashyap Krishnasamy, Anne Limbourg, Christine Häger, Tamar Kapanadze, Chieko Ishifune, Rabea Hinkel, Freddy Radtke, Lothar J. Strobl, Ursula Zimber-Strobl, L. Christian Napp, Johann Bauersachs, Hermann Haller, Koji Yasutomo, Christian Kupatt, Kenneth M. Murphy, Ralf H. Adams, Christian Weber, Florian P. Limbourg |
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| Přispěvatelé: | Biochemie, RS: CARIM - R3.07 - Structure-function analysis of the chemokine interactome for therapeutic targeting and imaging in atherosclerosis, RS: CARIM - R1.01 - Blood proteins & engineering, Pathology |
| Předmět: |
0301 basic medicine
Male Myeloid Science Population Notch signaling pathway General Physics and Astronomy Spleen Bone Marrow Cells Cell fate determination Biology GPI-Linked Proteins General Biochemistry Genetics and Molecular Biology Monocytes 03 medical and health sciences Mice In vivo MD Multidisciplinary medicine Animals Antigens Ly Humans Receptor Notch2 education Cells Cultured Mice Knockout education.field_of_study Multidisciplinary Monocyte Calcium-Binding Proteins Receptors IgG Endothelial Cells Cell Differentiation General Chemistry Adoptive Transfer Corrigenda Healthy Volunteers Recombinant Proteins Cell biology 030104 developmental biology medicine.anatomical_structure Intercellular Signaling Peptides and Proteins Bone marrow Signal Transduction |
| Zdroj: | Nat. Commun. 7:12597 (2016) Nature Communications Nature Communications, 7:12597. Nature Publishing Group Nature Communications, Vol 7, Iss 1, Pp 1-15 (2016) Nature communications, 7. Nature Publishing Group |
| ISSN: | 2041-1723 |
| Popis: | A population of monocytes, known as Ly6Clo monocytes, patrol blood vessels by crawling along the vascular endothelium. Here we show that endothelial cells control their origin through Notch signalling. Using combinations of conditional genetic deletion strategies and cell-fate tracking experiments we show that Notch2 regulates conversion of Ly6Chi monocytes into Ly6Clo monocytes in vivo and in vitro, thereby regulating monocyte cell fate under steady-state conditions. This process is controlled by Notch ligand delta-like 1 (Dll1) expressed by a population of endothelial cells that constitute distinct vascular niches in the bone marrow and spleen in vivo, while culture on recombinant DLL1 induces monocyte conversion in vitro. Thus, blood vessels regulate monocyte conversion, a form of committed myeloid cell fate regulation. |
| Databáze: | OpenAIRE |
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