High-mobility group AT-hook protein 2 expression and its prognostic significance in MGMT methylated and unmethylated glioblastoma
| Autor: | Anne Schänzer, Marcus A Weigand, Florian Uhle, Malgorzata Kolodziej, Marco Stein, Eberhard Uhl, Marcus H.T. Reinges, Frank P. Schwarm, Cornelia Weischer, Till Acker |
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| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Adult Male Cancer Research Methyltransferase Kaplan-Meier Estimate Biology Disease-Free Survival 03 medical and health sciences O(6)-Methylguanine-DNA Methyltransferase 0302 clinical medicine medicine Humans Promoter Regions Genetic Survival analysis Aged Regulation of gene expression HMGA2 Protein HMGA O-6-methylguanine-DNA methyltransferase Cancer Methylation DNA Methylation Middle Aged medicine.disease Prognosis Gene Expression Regulation Neoplastic 030104 developmental biology Oncology 030220 oncology & carcinogenesis DNA methylation Cancer research Female Glioblastoma |
| Zdroj: | International journal of oncology. 48(4) |
| ISSN: | 1791-2423 |
| Popis: | High-mobility group AT-hook protein 2 (HMGA 2) is a transcription factor associated with malignancy and poor prognosis in a variety of human cancers. We correlated HMGA 2 expression with clinical parameters, survival, and O-6-methylguanine-DNA methyltransferase methylation status (MGMT) in glioblastoma patients. HMGA 2 expression was determined by performing quantitative real-time polymerase chain reaction (qPCR) and immunohistochemistry (IHC) in 44 glioblastoma patients and 5 non-tumorous brain specimens as controls. Gene expression levels of MGMT methylated vs. unmethylated patients, and gene expression levels between patient groups, both for qPCR and IHC data were compared using the Mann-Whitney U test. The relationship between HMGA 2 expression, progression-free survival and overall survival was analyzed using the Kaplan-Meier method and the log-rank test. P-values of |
| Databáze: | OpenAIRE |
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