Invertase Proteinaceous Inhibitor ofCyphomandra BetaceaSendt Fruits
| Autor: | R. M. OrdónTez, María Inés Isla, Marta Amelia Vattuone, Antonio Rodolfo Sampietro |
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| Rok vydání: | 2000 |
| Předmět: |
Sucrose
Glycoside Hydrolases Ipomoea Biochemistry Epitope Lycopersicon Cell wall Lectins Enzyme Inhibitors Cyphomandra Plant Proteins Dose-Response Relationship Drug beta-Fructofuranosidase biology food and beverages Lectin Hydrogen-Ion Concentration biology.organism_classification Molecular Weight Invertase Fruit biology.protein Molecular Medicine Electrophoresis Polyacrylamide Gel Plant Lectins Solanaceae |
| Zdroj: | Journal of Enzyme Inhibition. 15:583-596 |
| ISSN: | 8755-5093 |
| DOI: | 10.3109/14756360009040712 |
| Popis: | This work describes a new invertase proteinaceous inhibitor from Cyphomandra betacea Sendt. (tomate de arbol) fruits. The proteinaceous inhibitor was isolated and purified from a cell wall preparation. The pH stability, kinetics of the inhibition of the C. betacea invertase, inhibition of several higher plant invertases and lectin nature of the inhibitor were studied. The inhibitor structure involves a single polypeptide (Mr = 19000), as shown by gel filtration and SDS-PAGE determinations. N-terminal aminoacid sequence was determined. The properties and some structural features of the inhibitor are compared with the proteinaceous inhibitors from several plant species (Beta vulgaris L., Ipomoea batatas L. and Lycopersicon esculentum Mill.). All these inhibitors share lectinic properties, some common epitopes, some aminoacid sequences and a certain lack of specificity towards invertases of different species, genera and even plant family. In consequence, the inhibitors appear to belong to the same lectin family. It is now known that some lectins are part of the defence mechanism of higher plants against fungi and bacteria and this is a probable role of the proteinaceous inhibitors. |
| Databáze: | OpenAIRE |
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