A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates
| Autor: | Bony De Kumar, Charles K. Kaufman, Hugo J. Parker, Robb Krumlauf, Karin D. Prummel, Christopher P. Hess, Leanne M. Wiedemann, Marianne E. Bronner, Christian Mosimann, Stephen A. Green |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures Science General Physics and Astronomy Hindbrain 02 engineering and technology General Biochemistry Genetics and Molecular Biology Article Cell Line Animals Genetically Modified 03 medical and health sciences Mice biology.animal Animals Enhancer Hox gene lcsh:Science Zebrafish Conserved Sequence Regulation of gene expression Homeodomain Proteins Multidisciplinary Binding Sites biology Lamprey Genes Homeobox Neural crest Vertebrate Gene Expression Regulation Developmental Lampreys Mouse Embryonic Stem Cells General Chemistry 021001 nanoscience & nanotechnology biology.organism_classification 030104 developmental biology Enhancer Elements Genetic Evolutionary biology Neural Crest Vertebrates embryonic structures lcsh:Q 0210 nano-technology Sequence Alignment |
| Zdroj: | Nature Communications, Vol 10, Iss 1, Pp 1-15 (2019) Nature Communications |
| ISSN: | 2041-1723 |
| Popis: | In jawed vertebrates (gnathostomes), Hox genes play an important role in patterning head and jaw formation, but mechanisms coupling Hox genes to neural crest (NC) are unknown. Here we use cross-species regulatory comparisons between gnathostomes and lamprey, a jawless extant vertebrate, to investigate conserved ancestral mechanisms regulating Hox2 genes in NC. Gnathostome Hoxa2 and Hoxb2 NC enhancers mediate equivalent NC expression in lamprey and gnathostomes, revealing ancient conservation of Hox upstream regulatory components in NC. In characterizing a lamprey hoxα2 NC/hindbrain enhancer, we identify essential Meis, Pbx, and Hox binding sites that are functionally conserved within Hoxa2/Hoxb2 NC enhancers. This suggests that the lamprey hoxα2 enhancer retains ancestral activity and that Hoxa2/Hoxb2 NC enhancers are ancient paralogues, which diverged in hindbrain and NC activities. This identifies an ancestral mechanism for Hox2 NC regulation involving a Hox-TALE regulatory circuit, potentiated by inputs from Meis and Pbx proteins and Hox auto-/cross-regulatory interactions. Mechanisms coupling Hox genes to neural crest are largely unknown. Here, the authors use cross species regulatory comparisons between the Hox2 genes of jawed vertebrates and lamprey, a jawless vertebrate, finding a conserved ancestral mechanism for Hox2 neural crest regulation. |
| Databáze: | OpenAIRE |
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