Isoproterenol facilitates GABAergic autapses in fast-spiking cells of rat insular cortex
| Autor: | Kurando Suga |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Cerebral Cortex
Male Interneuron Isoproterenol Action Potentials Anatomy Neurotransmission Biology Insular cortex Inhibitory postsynaptic potential Rats Synapse medicine.anatomical_structure Cerebral cortex medicine GABAergic Animals Autapse Female General Dentistry Neuroscience gamma-Aminobutyric Acid |
| Zdroj: | Journal of oral science. 56(1) |
| ISSN: | 1880-4926 |
| Popis: | In the cerebral cortex, fast-spiking (FS) cells are the principal GABAergic interneurons and potently suppress neural activity in targeting neurons. Some FS neurons make synaptic contacts with themselves. Such synapses are called autapses and contribute to self-inhibition of FS neural activity. β-Adrenoceptors have a crucial role in regulating GABAergic synaptic inputs from FS cells to pyra- midal (Pyr) cells; however, the β-adrenergic functions on FS autapses are unknown. To determine how the β-adrenoceptor agonist isoproterenol modulates inhibitory synaptic transmission in the autapses of FS cells, paired whole-cell patch-clamp recordings were obtained from FS and Pyr cells in layer V of rat insular cortex. Previous studies found that isopro- terenol (100 μM) had pleiotropic effects on unitary inhibitory postsynaptic currents (uIPSCs) in FSaPyr connections, whereas autapses in FS cells were always facilitated by isoproterenol. Facilitation of autapses by isoproterenol was accompanied by decreases in the paired-pulse ratio of second to first uIPSC ampli- tudes and the coefficient of variation of the uIPSC amplitude, which suggests that β-adrenergic facilita- tion is likely mediated by presynaptic mechanisms. The discrepancy between isoproterenol-induced modulation of uIPSCs in FS autapses and in FSaPyr connections may reflect the presence of different presynaptic mechanisms of GABA release in each synapse. (J Oral Sci 56, 41-47, 2014) |
| Databáze: | OpenAIRE |
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