The selectable antibiotic marker, tetA(C), increases Pseudomonas aeruginosa susceptibility to the herbicide/superoxide generator, paraquat
| Autor: | Skorn Mongkolsuk, Panithi Leesukon, Tatsanee Chuchue, Nisanart Charoenlap, Wilaiwan Wirathorn |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Paraquat
Gram-negative bacteria medicine.disease_cause Biochemistry Microbiology Antiporters chemistry.chemical_compound Bacterial Proteins Superoxides polycyclic compounds Genetics medicine Molecular Biology Selectable marker biology Herbicides Pseudomonas aeruginosa General Medicine Agrobacterium tumefaciens biochemical phenomena metabolism and nutrition biology.organism_classification Xanthomonas campestris chemistry Salmonella enterica Oxidation-Reduction Bacteria |
| Zdroj: | Archives of Microbiology. 195:671-674 |
| ISSN: | 1432-072X 0302-8933 |
| DOI: | 10.1007/s00203-013-0913-7 |
| Popis: | The presence of the widely used selectable antibiotic marker, tetA(C), unexpectedly increased the sensitivity of Pseudomonas aeruginosa PAO1 to the superoxide-generating herbicide, paraquat. A DNA fragment spanning the first 99 amino acids of TetA(C) was sufficient to confer paraquat sensitivity. The TetA(C)-induced paraquat sensitive phenotype was observed in other Gram-negative bacteria such as Agrobacterium tumefaciens, Salmonella enterica ser. Typhimurium and Xanthomonas campestris suggesting that this is a general property of tetA(C). This finding serves as a cautionary note for those using tetA(C) as a selectable marker for genetic manipulations in studies using paraquat either as a superoxide stress generator or a redox cycling drug. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink