Identification and Cloning in Yeast Artificial Chromosomes of a Region of Elevated Loss of Heterozygosity on Chromosome 1p31.1 in Human Breast Cancer
| Autor: | David Jones, Jennifer Varley, Louise A James, Nigel Hoggard, Bill Brintnell, Erika L D Mitchell, Yvonne Hey, J Weissenbach |
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| Rok vydání: | 1995 |
| Předmět: |
Genetics
Yeast artificial chromosome Heterozygote congenital hereditary and neonatal diseases and abnormalities Base Sequence Contig Molecular Sequence Data Chromosome Mapping Chromosome Breast Neoplasms Biology Loss of heterozygosity Restriction map Gene mapping Chromosomes Human Pair 1 Genetic marker Allelic Imbalance Humans Chromosome Deletion Cloning Molecular Chromosomes Artificial Yeast In Situ Hybridization Fluorescence DNA Primers Sequence Tagged Sites |
| Zdroj: | Genomics. 30:233-243 |
| ISSN: | 0888-7543 |
| Popis: | We have mapped a region of high loss of heterozygosity in breast cancer to a 2-cM interval between the loci D1S430 and D1S465 on chromosome 1p31.1. This region shows allelic imbalance in around 60% of breast tumors. As part of a strategy to clone the target gene(s) within this interval, we have generated a yeast artificial chromosome contig spanning over 7 Mb. YACs from the CEPH and Zeneca (formerly ICI) libraries have been obtained by screening with PCR-based STSs from the region for both previously identified loci and newly isolated STSs. The YACs have been assembled into a contig by a combination of approaches, including analysis of their STS content, generation of new STSs from the ends of key YACs, and long-range restriction mapping. These YAC clones provide the basis for complete characterization of the region of high loss in breast cancer and for the ultimate identification of the target gene(s). |
| Databáze: | OpenAIRE |
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