Chemical Profiling of Volatile Organic Compounds in the Headspace of Algal Cultures as Early Biomarkers of Algal Pond Crashes
| Autor: | James D. Jaryenneh, Pamela Lane, Matthew W. Moorman, Carolyn L. Fisher, A. Daniel Jones, Kristen L. Reese, Todd W. Lane, Matthias Frank |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine Rotifera lcsh:Medicine Rotifer Carotenoid degradation 01 natural sciences Article Applied microbiology 03 medical and health sciences Algae parasitic diseases Animals Ponds lcsh:Science Volatile Organic Compounds Multidisciplinary Ecology Environmental Biomarkers biology Small molecules fungi lcsh:R Algal growth Eutrophication Brachionus biology.organism_classification Good Health and Well Being 030104 developmental biology Environmental chemistry Environmental science lcsh:Q Biodiesel Biomarkers 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) Scientific reports, vol 9, iss 1 Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-50125-z |
| Popis: | Algae ponds used in industrial biomass production are susceptible to pathogen or grazer infestation, resulting in pond crashes with high economic costs. Current methods to monitor and mitigate unhealthy ponds are hindered by a lack of early indicators that precede culture crash. We used solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS) to identify volatiles emitted from healthy and rotifer infested cultures of Microchloropsis salina. After 48 hours of algal growth, marine rotifers, Brachionus plicatilis, were added to the algae cultures and volatile organic compounds (VOC) were sampled from the headspace using SPME fibers. A GC-MS approach was used in an untargeted analysis of VOCs, followed by preliminary identification. The addition of B. plicatilis to healthy cultures of M. salina resulted in decreased algal cell numbers, relative to uninfected controls, and generated trans-β-ionone and β-cyclocitral, which were attributed to carotenoid degradation. The abundances of the carotenoid-derived VOCs increased with rotifer consumption of algae. Our results indicate that specific VOCs released by infected algae cultures may be early indicators for impending pond crashes, providing a useful tool to monitor algal biomass production and pond crash prevention. |
| Databáze: | OpenAIRE |
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