Sequence analysis of a monoclonal antibody specific for the preS2 region of hepatitis B surface antigen, and the cloning, expression and characterisation of its single-chain Fv construction
| Autor: | M.-M. Riottot, Maria Passafiume, Brigitte Vulliez-Le Normand, Graham A. Bentley |
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| Rok vydání: | 1998 |
| Předmět: |
Hepatitis B virus
HBsAg DNA Complementary medicine.drug_class Molecular Sequence Data Biophysics Antibody engineering Monoclonal antibody Immunoglobulin light chain Binding Competitive Biochemistry Single chain Fv fragment Epitope law.invention PreS2 Antigen Antibody Specificity Structural Biology law Genetics medicine Amino Acid Sequence c-myc tag Cloning Molecular Protein Precursors Immunoglobulin Fragments Molecular Biology Hepatitis B Surface Antigens Base Sequence biology Chemistry Antibodies Monoclonal Cell Biology Virology Molecular biology Recombinant DNA biology.protein Paratope Antibody |
| Zdroj: | FEBS Letters. 441:407-412 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/s0014-5793(98)01581-6 |
| Popis: | The nucleotide sequence of the monoclonal antibody F124, specific for the preS2 region of the surface antigen of hepatitis B virus, has been determined and an single-chain Fv fragment (scFv) recombinant construction has been cloned and expressed into the periplasmic region of Escherichia coli. The recombinant antibody fragment contains a (Gly4Ser)3 linker connecting the C-terminus of the heavy chain variable region (VH) domain to the N-terminus of the light chain variable region (VL) domain. A 23-residue peptide segment, containing a c-myc marker for immunochemical detection of the scFv and hexahistidine tag to facilitate its purification, was added C-terminal to the VL domain. The scFv mimics the antibody in binding to the native antigen in the form of recombinant hepatitis B surface antigen (HBsAg) particles as well as to peptide fragments carrying the viral epitope. |
| Databáze: | OpenAIRE |
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