New Insights into the Ultrastructure of Bioapatite After Partial Dissolution: Based on Whale Rostrum, the Densest Bone
| Autor: | Zhen Li, Michael Yue, Lingyi Tang, Li Zhang, Mu Su, Da Tian |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Morphology (linguistics)
Mineral Materials science Layer by layer Whales Rostrum 02 engineering and technology Microscopy Atomic Force 010502 geochemistry & geophysics 021001 nanoscience & nanotechnology 01 natural sciences Bone and Bones Chemical engineering Apatites Ultrastructure Animals Collagen Crystallite Crystallization 0210 nano-technology High-resolution transmission electron microscopy Instrumentation Dissolution 0105 earth and related environmental sciences |
| Zdroj: | Microscopy and Microanalysis. 25:1323-1330 |
| ISSN: | 1435-8115 1431-9276 |
| DOI: | 10.1017/s1431927619015022 |
| Popis: | Mineral particles in bone are interlaced with collagen fibrils, hindering the investigation of bioapatite crystallites (BAp). This study utilized a special whale rostrum (the most highly mineralized bone ever recorded) to measure the crystallites of bone BAp via long-term dissolution in water. The BAp in the rostrum has a low solubility (6.7 ppm Ca and 3.8 ppm P after 150 days dissolution) as well as in normal bones, which leads to its Ksp value of ~10−53. Atomic force microscopy results show tightly compacted mineral crystallites and confirm the low amount of collagen in the rostrum. Additionally, the mineral crystallites demonstrate irregular plate-like shapes with variable sizes. The small crystallites (~11 × 24 nm) are easily detached from BAp prisms, compared with the large crystallites (~50 nm). Moreover, various orientations of crystallites are observed on the edge of the prisms, which suggest a random direction of mineral growth. Furthermore, these plate-like crystallites prefer to be stacked layer by layer under weak regulation from collagen. The morphology of rostrum after dissolution provides new insights into the actual morphology of BAp crystallites. |
| Databáze: | OpenAIRE |
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