Enumeration of virulent Yersinia enterocolitica colonies by DNA colony hybridization using alkaline treatment and paper filters
| Autor: | J A Jagow, Walter E. Hill |
|---|---|
| Rok vydání: | 1988 |
| Předmět: |
DNA
Bacterial Paper Filter paper DNA–DNA hybridization Nucleic Acid Hybridization Virulence Cell Biology Hydrogen-Ion Concentration Biology biology.organism_classification Enterobacteriaceae Microbiology chemistry.chemical_compound chemistry Food Microbiology Enumeration Autoradiography Yersinia enterocolitica Phosphorus Radioisotopes Molecular Biology DNA Bacteria |
| Zdroj: | Molecular and Cellular Probes. 2:189-195 |
| ISSN: | 0890-8508 |
| DOI: | 10.1016/0890-8508(88)90003-5 |
| Popis: | The efficiency of enumerating virulent Yersinia enterocolitica colonies was determined for 10 artificially contaminated foods by autoradiography after DNA colony hybridization using a 32 P-labelled genetic probe and paper filters. Enumeration efficiency, which ranged from 0–98% (average 25 ± 31% standard deviation), improved (42–138%, average 83 ± 27% standard deviation) after enrichment with 0·5% KOH-0·5% NaCl. These efficiencies are equivalent to those obtained with nitrocellulose filters. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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