Upregulation of centromere protein�M promotes tumorigenesis: A potential predictive target for cancer in humans
Autor: | Wenfeng Yu, Ting Zhang, Peng Ren, Ying Liu |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Cancer Research centromere protein M Carcinogenesis Breast Neoplasms Cell Cycle Proteins Kaplan-Meier Estimate Biology Transfection medicine.disease_cause Biochemistry 03 medical and health sciences breast cancer 0302 clinical medicine Centromere protein M Biomarkers Tumor Genetics medicine Humans Gene Regulatory Networks Protein Interaction Maps RNA Messenger Molecular Biology Chromosome separation Oncogene Gene Expression Profiling Cell Cycle Cancer Articles Cell cycle Prognosis medicine.disease Molecular biology Up-Regulation Gene Expression Regulation Neoplastic Survival Rate Gene expression profiling 030104 developmental biology Oncology Terminal deoxynucleotidyl transferase 030220 oncology & carcinogenesis Mutation MCF-7 Cells Molecular Medicine Female |
Zdroj: | Molecular Medicine Reports |
ISSN: | 1791-3004 1791-2997 |
Popis: | Centromere protein M (CENPM), a protein required for chromosome separation, is involved in in mitosis. However, little has been reported about the roles of CENPM in various types of cancer. The present study identified that the mRNA expression levels of CENPM were significantly upregulated in 14 types of human cancer and identified a positive association between CENPM mRNA expression and patient mortality using the Oncomine, Gene Expression Profiling Interactive Analysis, Human Protein Atlas and Kaplan-Meier Plotter databases. A protein interaction network constructed with CENPM-interacting genes obtained from the cBioPortal demonstrated that nine genes participating in the cell cycle served key roles in the function of CENPM. Cell cycle analysis, reverse transcription-quantitative polymerase chain reaction, a Cell Counting Kit-8-based proliferation assay and a terminal deoxynucleotidyl transferase dUTP nick end labelling assay further revealed the tumorigenic and carcinogenic roles of CENPM in vitro. In addition, it was identified that the mRNA expression levels of five of the nine identified genes were significantly associated with CENPM in MCF7 cells and that CENPM was rarely mutated among various types of human cancer. In conclusion, the data from the present study revealed that CENPM exerted its pro-tumorigenic function by regulating cell cycle-associated protein expression and suggested that CENPM could be used as a prognostic marker for breast cancer. |
Databáze: | OpenAIRE |
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