Anti-p53 antibodies in patients with Barrett's esophagus or esophageal carcinoma can predate cancer diagnosis
| Autor: | R. F. Souza, M. C. Hollstein, S. J. Meltzer, A. Salabes, G. E. Trivers, H. M. Cawley, H. Bartsch, V. M. G. De Benedetti, William P. Bennett, B. D. Greenwald, J. Cottrell, K. R. Muehlbauer, L. Liang |
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| Rok vydání: | 1998 |
| Předmět: |
Adult
Male medicine.medical_specialty Pathology Esophageal Neoplasms Biology Adenocarcinoma Gastroenterology Antibodies Barrett Esophagus Internal medicine medicine Humans Esophagus Aged Hepatology Esophageal disease Cancer DNA Esophageal cancer Middle Aged medicine.disease Immunohistochemistry digestive system diseases medicine.anatomical_structure Epidermoid carcinoma Dysplasia Barrett's esophagus Mutation Carcinoma Squamous Cell Tumor Suppressor Protein p53 |
| Zdroj: | Gastroenterology. 115(1) |
| ISSN: | 0016-5085 |
| Popis: | We previously discovered anti-p53 antibodies predating a cancer diagnosis in subjects at increased risk for liver, lung, breast, and prostate cancer. Recently, we reported a significant correlation (P0.017) between p53 antibodies and p53 mutations in patients with late-stage esophageal carcinoma. Because others have reported p53 mutations and overexpression of p53 protein in Barrett's esophagus, we studied p53 antibodies in plasma of 88 serially endoscoped patients: 36 with Barrett's metaplasia, 23 with esophageal squamous cell carcinoma, 10 with esophageal adenocarcinoma, and 19 with esophagitis or normal esophagus.We used enzyme immunoassay, immunoblotting, and immunoprecipitation assays for p53 antibodies; polymerase chain reaction, denaturant gradient gel electrophoresis, and sequencing for p53 mutations; and immunohistochemistry for p53 protein.p53 antibodies were detected in 4 patients with Barrett's esophagus, including 1 with dysplasia that later progressed to adenocarcinoma, and in 10 cancer patients (P = 0.002) (8 squamous and 2 adenocarcinoma), 2 of whom (1 squamous, 1 adenocarcinoma) had antibodies before cancer was diagnosed. Other patient groups were too small for informative statistical analysis. Six antibody-positive cancer patients had p53 mutations, whereas 2 patients with cancer and 1 with Barrett's esophagus with antibodies had p53 protein overexpressed in esophageal tissues.Patients with Barrett's esophagus and esophageal cancer can develop p53 antibodies that may predate the clinical diagnosis of malignancy. |
| Databáze: | OpenAIRE |
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