Luteolin Stimulates Proliferation and Inhibits Late Differentiation of Primary Rat Calvarial Osteoblast Induced by High-dose Dexamethasone via Sema3A /NRP1/Pleixin A1
| Autor: | Li Zheng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Cell Pharmaceutical Science Dexamethasone Rats Sprague-Dawley chemistry.chemical_compound Internal medicine medicine Animals Luteolin Cell Proliferation Osteoblasts biology Plexin Cell Differentiation Semaphorin-3A Osteoblast Neuropilin-1 Rats medicine.anatomical_structure Endocrinology chemistry Apoptosis Osteocalcin biology.protein Collagenase Biotechnology medicine.drug |
| Zdroj: | Current Pharmaceutical Biotechnology. 22:1538-1545 |
| ISSN: | 1389-2010 |
| Popis: | Background: Although Semaphorin 3A (Sema3A)/ Neuropilin-1(NRP1)/Plexin A1 is one of the important targets in bone metabolism, few studies are performed on this target in the glucocorticoids- induced osteoporosis. Luteolin is a chemical component of Honeysuckle and it has various bioactivities. The effect of Luteolin on the glucocorticoids-induced osteoporosis (primary osteoblasts model) remain unknown. Objective: The aim of this study was to investigate the action of Sema3A/ NRP1/Plexin A1 in Luteolin- induced osteoprotection against high-dose Dexamethasone. Methods: The effect of Luteolin on the proliferation, late differentiation, and apoptosis of primary osteoblasts model of glucocorticoids-induced osteoporosis in vitro as well as the expression of Sema3A/ NRP1/Plexin A1 are investigated by using 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-Htetrazolium bromide, Cell Counting Kit-8, reverse transcription-quantitative real-time polymerase chain reaction, western bolting and so on. Results: Suckling SD rats’ calvarial osteoblasts were isolated and identified. The glucocorticoidsinduced primary osteoporosis cell model was established by 100 μM Dexamethasone in 48 h (P Conclusion: The thesis concludes that Luteolin promoted the proliferation of osteoblast and inhibited its apoptosis and late differentiation in this glucocorticoids-induced primary osteoporosis cell model. This function may be related to the expression of up-regulated Sema3A/NRP1/Plexin A1. Therefore, Luteolin may be a potential medicine for the glucocorticoids-induced osteoporosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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