Performance of the Luminex xTAG Respiratory Viral Panel Fast in a clinical laboratory setting
| Autor: | Maija Lappalainen, Heli Piiparinen, Pia Jokela, Eeva Auvinen, Laura Mannonen |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Rhinovirus
viruses RT-PCR Microarray Respiratory virus Biology Real-Time Polymerase Chain Reaction medicine.disease_cause Sensitivity and Specificity Article Virus 03 medical and health sciences Human metapneumovirus Nasopharynx Virology Multiplex polymerase chain reaction Influenza A virus medicine Humans Direct fluorescent assay xTAG RVP Respiratory system Respiratory Tract Infections Enterovirus 030304 developmental biology 0303 health sciences Clinical Laboratory Techniques Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology virus diseases biology.organism_classification 3. Good health Real-time polymerase chain reaction Molecular Diagnostic Techniques Virus Diseases Detection rate |
| Zdroj: | Journal of Virological Methods |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/j.jviromet.2012.03.015 |
| Popis: | Highlights ► The RVP Fast assay gave valid results for 99.2% of samples in a clinical laboratory setting. ► The detection rate of RVP Fast was superior to DFA and comparable to real-time RT-PCR. ► RVP Fast detected more EVs/RVs than real-time RT-PCR. ► The RVP Fast assay needs adjustment to also cover the InfA (H1N1)2009 virus. The aim of the study was to develop a real-time RT-PCR for the detection of enteroviruses (EVs) and rhinoviruses (RVs) and to assess the performance of the xTAG RVP Fast assay in comparison to a direct fluorescent assay (DFA), a real-time RT-PCR assay for the detection of respiratory syncytial virus (RSV) and human metapneumovirus (hMPV), and the EV/RV RT-PCR assay developed in this study. The performance of the RVP Fast assay was assessed in the analysis of 373 nasopharyngeal samples. For the viruses of the DFA panel, detection rates of 27.6% and 23.8% were obtained by RVP and DFA, respectively, in analysis of a set of 297 samples collected in 2009–2010. These results show statistically significant superiority of the RVP Fast assay (P = 0.049). For RSV, hMPV, EV, and RV, detection rates of 48.0% and 45.2% were achieved by RVP and RT-PCR, respectively. For individual targets, increased detection of EV/RV (P = 0.043) and decreased detection of influenza A virus (P = 0.004) by RVP in comparison to real-time RT-PCR was observed. The results of the present study imply the need to adjust the InfA component of the RVP Fast assay to also cover the InfA(H1N1) 2009 virus. |
| Databáze: | OpenAIRE |
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