Postconditioning Ozone Alleviates Ischemia-Reperfusion Injury and Enhances Flap Endurance in Rats
| Autor: | Ozkan Onal, Mustafa Yilmaz, Cagdas Elsurer, Merih Onal, Jale Bengi Celik, Öznur Kal, Omer Erdur, Nebil Selimoglu, Ender Erdogan |
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| Přispěvatelé: | Selçuk Üniversitesi, Tıp Fakültesi, Cerrahi Tıp Bilimleri Bölümü, Elsürer, Çağdaş, Önal, Merih, Erdur, Ömer |
| Rok vydání: | 2018 |
| Předmět: |
Male
antioxidant Ischemia surgical flaps 03 medical and health sciences 0302 clinical medicine medicine Animals Humans Rats Wistar Surgical Flaps Ischemic Postconditioning ischemia reperfusion injury business.industry Graft Survival apoptosis medicine.disease eye diseases Rats Disease Models Animal Oxidative Stress ozone Reperfusion Injury 030220 oncology & carcinogenesis Anesthesia 030211 gastroenterology & hepatology Surgery business Reperfusion injury Injections Intraperitoneal |
| Zdroj: | Journal of Investigative Surgery. 33:15-24 |
| ISSN: | 1521-0553 0894-1939 |
| DOI: | 10.1080/08941939.2018.1473901 |
| Popis: | PubMed: 30339503 Muscle-flap transferring is a routine approach utilized in reconstructive operations; however, flap morbidity is often a source of post-operative difficulty. Ischemia-Reperfusion Injury (IRI) is an important contributor to the viability of flaps after transferring. The goal of this research was for assess the probable useful impacts of ozone on flap survival in a rat muscle-flap design. Materials and Methods: We examined the effects of postconditioning ozone administration on viability of pedicled composite flaps. Twenty-eight Wistar rats were randomized into four groups: sham-operated (S), ischemia-reperfusion (IR), sham-operated + ozone (O), IR + ozone (IR + O), respectively. The animals were sacrificed on the eighth day. In a general histological evaluation, flap tissues were examined with a light microscope, and apoptotic cells were counted. The Apoptotic Index (AI) was then calculated. Flap-tissue samples were sent for analyses of malondialdehyde (MDA), catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and protein carbonyl (PCO), and blood samples were sent for analyses of Total Oxidant Score (TOS), and Total Antioxidant Capacity (TAC). Data were evaluated statistically using the Kruskal–Wallis test. Results: The histomorphometric score was remarkably greater in O (p =.002). The AI was greater in IR (p =.002). The antioxidant parameters values as regards SOD, GSH-Px, CAT, and TAC were found to be greater in O (p |
| Databáze: | OpenAIRE |
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