Chondromodulin I and pleiotrophin gene expression in bovine cartilage and epiphysis
| Autor: | Hazel Tapp, Prasanthi Govindraj, Azliyati Azizan, Peter J. Neame, Joanne U. Gaw |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Retinoic acid
Tretinoin Biology Pleiotrophin Polymerase Chain Reaction chemistry.chemical_compound Chondrocytes medicine Limb development Animals Northern blot RNA Messenger Growth Substances Molecular Biology Messenger RNA Cartilage Gene Expression Regulation Developmental Bovine Cartilage Cell biology medicine.anatomical_structure Biochemistry chemistry Epiphysis Cytokines Intercellular Signaling Peptides and Proteins Cattle Carrier Proteins Epiphyses |
| Zdroj: | Matrix biology : journal of the International Society for Matrix Biology. 19(6) |
| ISSN: | 0945-053X |
| Popis: | Pleiotrophin and chondromodulin-I are low molecular weight proteins that are abundant (20 μg/g tissue) in fetal cartilage and difficult to detect in adult cartilage. We characterized their gene and protein expression patterns to gain a better understanding of their roles in the regulation of limb development and growth. In order to compare and contrast the relative amounts of the respective mRNA species within the developing epiphysis, a competitive PCR assay was developed. The results showed that the mRNAs for both proteins were abundant in fetal cartilage and while present in adult cartilage, were at 20–60-fold lower levels. Northern blotting revealed gradients of mRNA for both of these proteins in growth plate cartilage, with the highest levels in the resting zone, and the lowest in the hypertrophic zone. In contrast to pleiotrophin, chondromodulin-1 is down-regulated by retinoic acid with a pattern of expression similar to collagen type II and link protein, and may play a more specific role than pleiotrophin in modulating the chondrocyte phenotype. |
| Databáze: | OpenAIRE |
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