Dysregulated homeostasis of acetylcholine levels in immune cells of RR-multiple sclerosis patients
| Autor: | Stefano Biagioni, Erica Costantini, Ada Maria Tata, Marcella Reale, Serena Ruggieri, Viviana Orlando, Sabrina Galizia, Chiara D'Angelo, Italo Porfilio, Marta Di Nicola, Maria Di Bari, Claudio Gasperini |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment multiple sclerosis acetylcholine acetylcholinesterase butyrylcholinesterase cytokines lcsh:Chemistry chemistry.chemical_compound 0302 clinical medicine lcsh:QH301-705.5 Spectroscopy Interleukin-18 General Medicine Middle Aged Interleukin-12 Acetylcholinesterase Computer Science Applications Cytokine Female medicine.symptom Acetylcholine medicine.drug Adult medicine.medical_specialty Inflammation Biology Peripheral blood mononuclear cell Article Catalysis Inorganic Chemistry Young Adult 03 medical and health sciences Multiple Sclerosis Relapsing-Remitting Immune system Internal medicine medicine Humans Physical and Theoretical Chemistry Molecular Biology Aged Tumor Necrosis Factor-alpha Organic Chemistry 030104 developmental biology Endocrinology lcsh:Biology (General) lcsh:QD1-999 chemistry Cholinergic 030217 neurology & neurosurgery Homeostasis |
| Zdroj: | International Journal of Molecular Sciences; Volume 17; Issue 12; Pages: 2009 International Journal of Molecular Sciences, Vol 17, Iss 12, p 2009 (2016) International Journal of Molecular Sciences |
| Popis: | Multiple sclerosis (MS) is characterized by pro-inflammatory cytokine production. Acetylcholine (ACh) contributes to the modulation of central and peripheral inflammation. We studied the homeostasis of the cholinergic system in relation to cytokine levels in immune cells and sera of relapsing remitting-MS (RR-MS) patients. We demonstrated that lower ACh levels in serum of RR-MS patients were inversely correlated with the increased activity of the hydrolyzing enzymes acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE). Interestingly, the expression of the ACh biosynthetic enzyme and the protein carriers involved in non-vesicular ACh release were found overexpressed in peripheral blood mononuclear cells of MS patients. The inflammatory state of the MS patients was confirmed by increased levels of TNFα, IL-12/IL-23p40, IL-18. The lower circulating ACh levels in sera of MS patients are dependent on the higher activity of cholinergic hydrolyzing enzymes. The smaller ratio of ACh to TNFα, IL-12/IL-23p40 and IL-18 in MS patients, with respect to healthy donors (HD), is indicative of an inflammatory environment probably related to the alteration of cholinergic system homeostasis. |
| Databáze: | OpenAIRE |
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