Fine structural characterization of sulodexide
| Autor: | Giulia Risi, Elena Urso, Noemi Veraldi, Marco Guerrini, Sabrina Bertini, Donata Bensi, Antonella Bisio |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Magnetic Resonance Spectroscopy Clinical Biochemistry Pharmaceutical Science Dermatan Sulfate Oligosaccharides Heparinoid 030204 cardiovascular system & hematology Chemical Fractionation Dermatan sulfate Mass Spectrometry Analytical Chemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Drug Discovery medicine Heparinoids Spectroscopy Chromatography High Pressure Liquid Glycosaminoglycans Chromatography Molecular Structure Heparin Antithrombin Sulodexide Characterization (materials science) Molecular Weight 030104 developmental biology chemistry medicine.drug |
| Zdroj: | Journal of pharmaceutical and biomedical analysis. 156 |
| ISSN: | 1873-264X |
| Popis: | Sulodexide is a heparinoid which combines the properties of its components heparin and dermatan sulfate and is used not only for the prophylaxis and treatment of thromboembolic diseases but also for the treatment of diabetic nephropathy. Despite many clinical studies have been conducted to investigate its activity and safety, no data are available on the fine chemical characterization of its components. In this work, the in-depth investigation on the structural features of both the whole mixture and the isolated components was accomplished, involving the analysis of molecular weight distribution and of their mono, di and oligosaccharide composition by HP-SEC/TDA, 2D-NMR and HPLC-MS techniques. Moreover, also the separation of fractions endowed of graded affinity to antithrombin was achieved followed again by detailed structural analysis. The combination of different techniques permits to profile in depth the structural features of such a drug and offers a useful tool for possible analysis of batch production. |
| Databáze: | OpenAIRE |
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