Detection of the degradation products of bradykinin by enzyme immunoassays as markers for the release of kinin in vivo
| Autor: | Sunahara Noriyuki, Katori Makoto, Majima Masataka, Harada Yoshiteru |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Male
Captopril Time Factors High-molecular-weight kininogen Molecular Sequence Data Bradykinin Peptide Carrageenan Sensitivity and Specificity Biochemistry Immunoenzyme Techniques Rats Sprague-Dawley chemistry.chemical_compound In vivo Animals Amino Acid Sequence Bovine serum albumin Pleurisy Trypsin Inhibitor Bowman-Birk Soybean Pharmacology chemistry.chemical_classification biology Kinin Peptide Fragments Rats Pleural Effusion Enzyme chemistry biology.protein Biomarkers |
| Zdroj: | Biochemical Pharmacology. 45:559-567 |
| ISSN: | 0006-2952 |
| DOI: | 10.1016/0006-2952(93)90127-i |
| Popis: | We developed an enzyme immunoassay (EIA) specific for Arg1-Pro2-Pro3-Gly4-Phe5 ([1-5]-BK) for determination of the levels of this peptide in biological fluids. Previously developed EIAs for bradykinin (BK) and for des-Phe8-Arg9-BK ([1-7]BK) were also used. Incubation of rat plasma with glass powder resulted in the transient appearance of BK. A degradation product, [1-7]BK, could be detected in the incubation mixture for a longer period of time. When compared with BK and [1-7]BK, a larger amount of [1-5]BK was detectable even longer. In carrageenan-induced pleurisy in rats, which was associated with a peak rate of plasma exudation 5 hr after administration of carrageenan, BK was undetectable (160 pg/rat) in the pleural exudates. By contrast, [1-7]BK was detectable over the entire course of the inflammatory response. A larger amount of [1-5]BK was detectable. The peak level of [1-5]BK was 6050 +/- 1050 pg/rat, 5 hr after administration of carrageenan. Inhibition of the generation of BK by intrapleural administration of soy bean trypsin inhibitor (0.3 mg/rat) 30 min before collection of pleural fluid resulted in significant reductions in the levels of both [1-7]BK (by 51-65%) and [1-5]BK (by 63-79%) in the exudates 3, 7 and 19 hr after administration of carrageenan. Intraperitoneal administration of captopril (10 mg/kg) caused a marked reduction (by 98%) in levels of [1-5]BK in exudates 3 hr after administration of carrageenan. The reduction was accompanied by an increase in the level of BK up to 1250% of that in untreated rats. These results indicate that the newly developed EIA for [1-5]BK might be a useful tool for verifying the release of kinin in vivo. |
| Databáze: | OpenAIRE |
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