Tbx3 represses PTEN and is over-expressed in head and neck squamous cell carcinoma

Autor: Ugur Yavuzer, Durmuş Burgucu, Kenan Güney, Irem Hicran Ozbudak, Duygu Sahinturk, Deniz Ozel, Gulay Ozbilim
Rok vydání: 2012
Předmět:
PTEN
Cancer Research
Transcription
Genetic
Immunoblotting
Repressor
Transfection
lcsh:RC254-282
Flow cytometry
HeLa
03 medical and health sciences
0302 clinical medicine
Squamous cell carcinoma
Biomarkers
Tumor
Genetics
medicine
Humans
Promoter Regions
Genetic
Cancer
030304 developmental biology
0303 health sciences
biology
medicine.diagnostic_test
Oncogene
Reverse Transcriptase Polymerase Chain Reaction
HEK 293 cells
PTEN Phosphohydrolase
Tbx3
Flow Cytometry
lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens
biology.organism_classification
medicine.disease
Immunohistochemistry
Molecular biology
Head and neck squamous-cell carcinoma
3. Good health
Gene Expression Regulation
Neoplastic
stomatognathic diseases
HEK293 Cells
Real-time polymerase chain reaction
Oncology
Head and Neck Neoplasms
030220 oncology & carcinogenesis
Carcinoma
Squamous Cell
Cancer research
biology.protein
T-Box Domain Proteins
Research Article
HeLa Cells
Zdroj: BMC Cancer, Vol 12, Iss 1, p 481 (2012)
BMC Cancer
ISSN: 1471-2407
DOI: 10.1186/1471-2407-12-481
Popis: Background Despite advances in diagnostic and treatment strategies, head and neck squamous cell cancer (HNSCC) constitutes one of the worst cancer types in terms of prognosis. PTEN is one of the tumour suppressors whose expression and/or activity have been found to be reduced in HNSCC, with rather low rates of mutations within the PTEN gene (6-8%). We reasoned that low expression levels of PTEN might be due to a transcriptional repression governed by an oncogene. Tbx2 and Tbx3, both of which are transcriptional repressors, have been found to be amplified or over-expressed in various cancer types. Thus, we hypothesize that Tbx3 may be over expressed in HNSCC and may repress PTEN, thus leading to cancer formation and/or progression. Methods Using immunohistochemistry and quantitative PCR (qPCR), protein and mRNA levels of PTEN and Tbx3 were identified in samples excised from cancerous and adjacent normal tissues from 33 patients who were diagnosed with HNSCC. In addition, HeLa and HEK cell lines were transfected with a Tbx3 expressing plasmid and endogenous PTEN mRNA and protein levels were determined via qPCR and flow cytometry. Transcription assays were performed to demonstrate effects of Tbx3 on PTEN promoter activity. Mann–Whitney, Spearman’s Correlation and Wilcoxon signed-rank tests were used to analyze the data. Results We demonstrate that in HNSCC samples, Tbx3 mRNA levels are increased with respect to their normal tissue counterparts (p Conclusions We show that Tbx3 is up-regulated in tissue samples of HNSCC patients and that Tbx3 represses PTEN transcription. Thus, our data not only reveals a new mechanism that may be important in cancer formation, but also suggests that Tbx3 can be used as a potential biomarker in cancer.
Databáze: OpenAIRE
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