Serological survey of Babesia bovis, Babesia bigemina, and Anaplasma marginale antibodies in cattle from the semi-arid region of the state of Bahia, Brazil, by enzyme-linked immunosorbent assays
| Autor: | Elaine P. S. Melo, Raul H. Kessler, Carlos F Menk, Silvia L Barros, Maria Angela Ornelas de Almeida, Cláudio R. Madruga, Flábio R. Araújo |
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| Rok vydání: | 2005 |
| Předmět: |
Microbiology (medical)
Anaplasmosis lcsh:Arctic medicine. Tropical medicine Babesia bigemina lcsh:RC955-962 prevalence semi-arid lcsh:QR1-502 Antibodies Protozoan Babesia Cattle Diseases Enzyme-Linked Immunosorbent Assay lcsh:Microbiology Serology Antigen Seroepidemiologic Studies Babesiosis parasitic diseases Prevalence Animals biology Babesia bovis biology.organism_classification Virology Antibodies Bacterial Anaplasma marginale Rickettsia biology.protein Enzootic ELISA Cattle Antibody Brazil |
| Zdroj: | Memórias do Instituto Oswaldo Cruz., Vol 100, Iss 6, Pp 513-517 (2005) Memórias do Instituto Oswaldo Cruz, Volume: 100, Issue: 6, Pages: 513-517, Published: OCT 2005 |
| ISSN: | 0074-0276 |
| Popis: | The objectives of this work were to determine the prevalence of Babesia bovis, Babesia bigemina, and Anaplasma marginale detecting antibodies in cattle raised in the semi-arid region of the state of Bahia, Brazil, through indirect enzyme linked immunosorbent assays (ELISA) and to compare the performances of indirect enzyme-linked immunosorbent assays with crude (I-ELISA-CrAnaAg) and recombinant major surface protein-5 (I-ELISA-MSP-5Ag), as antigens to detect antibodies against A. marginale. An stable enzootic area was found in Senhor do Bonfim and Euclides da Cunha for B. bovis that showed 86 and 95.5% of prevalence, respectively, and for B. bigemina with 90.8 and 91.3%. On the other hand, Uauá and Juazeiro, were characterized as enzootically unstable areas, since prevalences were: B. bovis - 63.7 and 56.4% and B. bigemina - 53 and 54.8%, respectively. The prevalence of A. marginale in the four municipalities was above 97% with I-ELISA-CrAnaAg and 94.8% with I-ELISA-MSP-5Ag which is an indication of stable enzootic condition for the rickettsia. The I-ELISA-CrAnaAg and I-ELISA-MSP-5Ag showed a highly significant association (r = 0.977), which means that both ELISA tests are suitable for epidemiological studies of A. marginale. |
| Databáze: | OpenAIRE |
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