Lawsone, a 2-hydroxy-1,4-naphthoquinone from Lawsonia inermis (henna), produces mitochondrial dysfunctions and triggers mitophagy in Saccharomyces cerevisiae
| Autor: | Marcos Antonio de Morais, Maise Gomes Queiroz, Manuella Maria Silva Santos, Mariza S. de Lima Silva, Mariana Rodrigues Xavier, Alexandre José da Silva Góes |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Saccharomyces cerevisiae Mutant Gene Expression Microbial Sensitivity Tests medicine.disease_cause Lawsone 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Genes Reporter Mitophagy Genetics medicine Molecular Biology biology Dose-Response Relationship Drug Molecular Structure Plant Extracts General Medicine biology.organism_classification Naphthoquinone Yeast Mitochondria Lawsonia Plant Oxidative Stress 030104 developmental biology chemistry Biochemistry 030220 oncology & carcinogenesis Intracellular Oxidative stress Naphthoquinones |
| Zdroj: | Molecular biology reports. 47(2) |
| ISSN: | 1573-4978 |
| Popis: | Lawsone is a natural naphthoquinone present in the henna leaf extract with several cytotoxic activities and used as precursor for synthesis of various pharmaceutical compounds. Its biological activities are thought to be the result of oxidative stress generated, although the hydroxy group at position C-2 in its structure tends to reduce its electrophilic potential. In view of lack of knowledge on its activity, the present work aimed to elucidate the biological effect of lawsone using the yeast Saccharomyces cerevisiae. In the model strain BY4741 it was defined 229 mmol/L as the minimal inhibitory concentration (MIC). Using 172 mmol/L as sub-MIC value it was observed that yap1 deletion mutant was sensitive to lawsone independent the presence of oxygen. Lawsone affected yeast growth in glycerol, indicating interference in the respiratory metabolism. Intracellular content of thiol groups did not indicate intensive oxidative stress and the presence of the anti-oxidant N-acetylcysteine (NAC) exacerbated lawsone toxicity. By analysing the sensitivity of atg mutant strains and the localization of GFP-Atg8 fusion protein, it was concluded that lawsone primarily produces mitochondrial malfunctioning, leading to indirect oxidative stress. It triggers the autophagic response that ultimately induces mitophagy. |
| Databáze: | OpenAIRE |
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