Miniaturization of multiplexed planar recombinant antibody arrays for serum protein profiling
| Autor: | Carl A.K. Borrebaeck, Nabil A. Amro, Michael Coen, Lennart Truedsson, Linn Petersson, Christer Wingren |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Miniaturization
Materials science Antibody microarray Clinical Biochemistry Protein Array Analysis Blood Proteins General Medicine Pharmacology and Toxicology Proteomics Multiplexing Molecular biology Antibodies Recombinant Proteins Analytical Chemistry Medical Laboratory Technology Nanolithography Proteome Humans Multiplex General Pharmacology Toxicology and Pharmaceutics DNA microarray Biomedical engineering |
| Zdroj: | Bioanalysis; 6(9), pp 1175-1185 (2014) |
| ISSN: | 1757-6180 |
| Popis: | Background: Antibody-based microarrays are a developing tool for high-throughput proteomics in health and disease. However, in order to enable global proteome profiling, novel miniaturized high-density antibody array formats must be developed. Results: In this proof-of-concept study, we have designed a miniaturized planar recombinant (single-chain Fragment variable). antibody array technology platform for multiplexed profiling of non-fractionated, directly labelled serum samples. The size of the individual spot features was reduced 225-times (78.5 μm2/spot) and the array density was increased 19-times (38,000 spots/cm2). These miniaturized, multiplexed arrays were produced, using a desktop nanofabrication system based on dip-pen nanolithography technology, and interfaced with a high-resolution fluorescent-based scanner. The reproducibility, sensitivity, specificity, and applicability of the set-up were demonstrated by profiling a set of well-characterized serum samples. Conclusion: The designed antibody array platform opens up new possibilities for large-scale, multiplex profiling of crude proteomes in a miniaturized fashion. |
| Databáze: | OpenAIRE |
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