Comparative neuronal differentiation of self-renewing neural progenitor cell lines obtained from human induced pluripotent stem cells
| Autor: | Elena Fusar Poli, Domenico Delia, Christopher Heise, Daniel Orellana, Giulia Bechi, Carlo Sala, Silvana Franceschetti, Renato Mantegazza, Massimo Mantegazza, Chiara Verpelli, Luigi Carlessi |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
Matrigel
medicine.diagnostic_test induced pluripotent stem cells postsynapse Neurodegeneration Biology Immunofluorescence medicine.disease In vitro Postsynapse lcsh:RC321-571 Technology Report Article Cellular and Molecular Neuroscience Electrophysiology nervous system Human neurons synapse formation medicine Progenitor cell Induced pluripotent stem cell Neuroscience lcsh:Neurosciences. Biological psychiatry. Neuropsychiatry neuronal differentiation |
| Zdroj: | Frontiers in Cellular Neuroscience Frontiers in Cellular Neuroscience, Vol 7 (2013) |
| ISSN: | 1662-5102 |
| Popis: | Most human neuronal disorders are associated with genetic alterations that cause defects in neuronal development and induce precocious neurodegeneration. In order to fully characterize the molecular mechanisms underlying the onset of these devastating diseases, it is important to establish in vitro models able to recapitulate the human pathology as closely as possible. Here we compared three different differentiation protocols for obtaining functional neurons from human induced pluripotent stem cells (hiPSCs): human neural progenitors (hNPs) obtained from hiPSCs were differentiated by co-culturing them with rat primary neurons, glial cells or simply by culturing them on matrigel in neuronal differentiation medium, and the differentiation level was compared using immunofluorescence, biochemical and electrophysiological methods. We show that the differentiated neurons displayed distinct maturation properties depending on the protocol used and the faster morphological and functional maturation was obtained when hNPs were co-cultured with rat primary neurons. |
| Databáze: | OpenAIRE |
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